domestic rabbit monoclonal (PSH12-64)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human Angiogenin matched pair antibodies Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725076) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein (HA210986) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA725077, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Angiogenin in HepG2, HT-29 and MCF7 cell culture supernatant. Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 2.5%, HT-29 cell culture supernatant 100% and MCF7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 19,596 pg/ml in HepG2 cell culture supernatant, 151 pg/ml in HT-29 cell culture supernatant and undetectable in MCF7 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-65)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human Angiogenin matched pair antibodies Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725076) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein (HA210986) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA725077, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Angiogenin in HepG2, HT-29 and MCF7 cell culture supernatant. Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 2.5%, HT-29 cell culture supernatant 100% and MCF7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 19,596 pg/ml in HepG2 cell culture supernatant, 151 pg/ml in HT-29 cell culture supernatant and undetectable in MCF7 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-65)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human Angiogenin matched pair antibodies Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725076) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein (HA210986) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA725077, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Angiogenin in HepG2, HT-29 and MCF7 cell culture supernatant. Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 2.5%, HT-29 cell culture supernatant 100% and MCF7 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 19,596 pg/ml in HepG2 cell culture supernatant, 151 pg/ml in HT-29 cell culture supernatant and undetectable in MCF7 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-65)
reactivity: human
conjugate: HRP
application: ELISA
reactivity: human
conjugate: HRP
application: ELISA
Sandwich ELISA analysis of human Angiogenin matched pair antibodies Capture: HA725076, Human Angiogenin Rabbit mAb [PSH12-64] Detector: HA725077, Human Angiogenin Rabbit mAb [PSH12-65] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725076) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Angiogenin protein (HA210986) starting from 2,500 pg/ml to 0 pg/ml and detect antibody (HA725077, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier