mouse monoclonal (7-C2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of PRMT5 on mouse kidney lysates using anti-PRMT5 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit monoclonal (ST51-06)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of PRMT5 on different lysates with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Raji cell lysate Lane 4: Jurkat cell lysate Lane 5: A431 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lane 8: bEnd.3 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: C2C12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-43) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling PRMT5 with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Western blot analysis of PRMT5 on different lysates with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Raji cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lane 6: A431 cell lysate Lane 7: COS-1 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: bEnd.3 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: C2C12 cell lysate Lane 12: C6 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-43) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (ST51-06)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of PRMT5 on different lysates with Rabbit anti-PRMT5 antibody (HA750174) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Raji cell lysate Lane 4: Jurkat cell lysate Lane 5: A431 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lane 8: bEnd.3 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: C2C12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750174) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling PRMT5 with Rabbit anti-PRMT5 antibody (HA750174) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRMT5 antibody (HA750174) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Western blot analysis of PRMT5 on different lysates with Rabbit anti-PRMT5 antibody (HA750174) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Raji cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lane 6: A431 cell lysate Lane 7: COS-1 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: bEnd.3 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: C2C12 cell lysate Lane 12: C6 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750174) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of PRMT5 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1204-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ICC staining of PSMA3 in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1204-3, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier