domestic rabbit monoclonal (JE40-17)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ANGPTL3 on different lysates with Rabbit anti-ANGPTL3 antibody (HA722388) at 1/1,000 dilution. Lane 1: Huh7 cell lysate Lane 2: HepG2 cell lysate Lane 3: A375 cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ANGPTL3 on different lysates with Rabbit anti-ANGPTL3 antibody (HA722388) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722388) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ANGPTL3 antibody (HA722388) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722388) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-39)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human ANGPTL3 matched pair antibodies Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39] Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725079) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ANGPTL3 protein protein starting from 25,000 pg/ml to 0 pg/ml and detect antibody (HA725080, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native ANGPTL3 in Human serum. Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39] Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40] Interpolated concentration of native ANGPTL3 was measured in duplicate at different sample concentrations and interpolated from the ANGPTL3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 262,537 pg/ml in Mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-40)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human ANGPTL3 matched pair antibodies Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39] Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725079) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ANGPTL3 protein protein starting from 25,000 pg/ml to 0 pg/ml and detect antibody (HA725080, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native ANGPTL3 in Human serum. Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39] Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40] Interpolated concentration of native ANGPTL3 was measured in duplicate at different sample concentrations and interpolated from the ANGPTL3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 262,537 pg/ml in Mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-40)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human ANGPTL3 matched pair antibodies Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39] Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725079) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ANGPTL3 protein protein starting from 25,000 pg/ml to 0 pg/ml and detect antibody (HA725080, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native ANGPTL3 in Human serum. Capture: HA725079, Human ANGPTL3 Rabbit mAb [PSH17-39] Detector: HA725080, Human ANGPTL3 Rabbit mAb [PSH17-40] Interpolated concentration of native ANGPTL3 was measured in duplicate at different sample concentrations and interpolated from the ANGPTL3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ANGPTL3 concentration was determined to be 262,537 pg/ml in Mouse serum.
quantity: 100μl
price: 409.00 USD
to the supplier