domestic rabbit monoclonal (PSH11-89)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human Siglec 9 matched pair antibodies Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723398, Siglec 9 Rabbit mAb [PSH11-90] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723397) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 9 protein (HA211074) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723398, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of human Siglec 9 matched pair antibodies Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723400, Siglec 9 Rabbit mAb [PSH11-91] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723397) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 9 protein (HA211074) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723400, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Siglec-9 in U937 and Jurkat extract samples based on a 1,000 µg/ml extract load. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723398, Siglec 9 Rabbit mAb [PSH11-90] The concentrations of Siglec-9 were measured in duplicates, interpolated from the Siglec-9 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 100% and Jurkat extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Siglec-9 concentration was determined to be 159.3 pg/ml in U937 extract and undetectable in Jurkat extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH11-90)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human Siglec 9 matched pair antibodies Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723398, Siglec 9 Rabbit mAb [PSH11-90] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723397) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 9 protein (HA211074) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723398, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Siglec-9 in U937 and Jurkat extract samples based on a 1,000 µg/ml extract load. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723398, Siglec 9 Rabbit mAb [PSH11-90] The concentrations of Siglec-9 were measured in duplicates, interpolated from the Siglec-9 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 100% and Jurkat extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Siglec-9 concentration was determined to be 159.3 pg/ml in U937 extract and undetectable in Jurkat extract.
Interpolated concentrations of spiked Siglec-9 in human cell culture media samples. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723398, Siglec 9 Rabbit mAb [PSH11-90] The concentrations of Siglec-9 were measured in duplicates, interpolated from the Siglec-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH11-90)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human Siglec 9 matched pair antibodies Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723398, Siglec 9 Rabbit mAb [PSH11-90] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723397) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 9 protein (HA211074) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723399B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Siglec-9 in U937 and Jurkat extract samples based on a 1,000 µg/ml extract load. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723398, Siglec 9 Rabbit mAb [PSH11-90] The concentrations of Siglec-9 were measured in duplicates, interpolated from the Siglec-9 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 100% and Jurkat extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Siglec-9 concentration was determined to be 159.3 pg/ml in U937 extract and undetectable in Jurkat extract.
Interpolated concentrations of spiked Siglec-9 in human cell culture media samples. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723398, Siglec 9 Rabbit mAb [PSH11-90] The concentrations of Siglec-9 were measured in duplicates, interpolated from the Siglec-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH11-91)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human Siglec 9 matched pair antibodies Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723400, Siglec 9 Rabbit mAb [PSH11-91] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723397) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 9 protein (HA211074) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723400, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Siglec-9 in U937 and Jurkat extract samples based on a 1,000 µg/ml extract load. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723400, Siglec 9 Rabbit mAb [PSH11-91] The concentrations of Siglec-9 were measured in duplicates, interpolated from the Siglec-9 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 100% and Jurkat extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Siglec-9 concentration was determined to be 168.5 pg/ml in U937 extract and undetectable in Jurkat extract.
Interpolated concentrations of spiked Siglec-9 in human cell culture media samples. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723400, Siglec 9 Rabbit mAb [PSH11-91] The concentrations of Siglec-9 were interpolated from the Siglec-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH11-91)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human Siglec 9 matched pair antibodies Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723400, Siglec 9 Rabbit mAb [PSH11-91] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723397) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Siglec 9 protein (HA211074) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723400, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Siglec-9 in U937 and Jurkat extract samples based on a 1,000 µg/ml extract load. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723400, Siglec 9 Rabbit mAb [PSH11-91] The concentrations of Siglec-9 were measured in duplicates, interpolated from the Siglec-9 standard curve and corrected for sample dilution. Undiluted samples are U937 extract 100% and Jurkat extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Siglec-9 concentration was determined to be 168.5 pg/ml in U937 extract and undetectable in Jurkat extract.
Interpolated concentrations of spiked Siglec-9 in human cell culture media samples. Capture: HA723397, Siglec 9 Rabbit mAb [PSH11-89] Detector: HA723400, Siglec 9 Rabbit mAb [PSH11-91] The concentrations of Siglec-9 were interpolated from the Siglec-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.
quantity: 100μl
price: 409.00 USD
to the supplier