domestic rabbit monoclonal (JM108-09)
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of Growth Hormone on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (ET1703-47, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 25 kDa Observed band size: 25 kDa
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Growth Hormone antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Growth Hormone antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-81)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human Growth Hormone matched pair antibodies Capture: HA725106, Human Growth Hormone Rabbit mAb [PSH12-81] Detector: HA725107, Human Growth Hormone Rabbit mAb [PSH12-82] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725106) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Growth Hormone protein (HA211027) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA725107, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Growth Hormone in human serum samples. Capture: HA725106, Human Growth Hormone Rabbit mAb [PSH12-81] Detector: HA725107, Human Growth Hormone Rabbit mAb [PSH12-82] Interpolated concentration of native Growth Hormone was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 1522 pg/mL in Human serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-82)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human Growth Hormone matched pair antibodies Capture: HA725106, Human Growth Hormone Rabbit mAb [PSH12-81] Detector: HA725107, Human Growth Hormone Rabbit mAb [PSH12-82] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725106) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Growth Hormone protein (HA211027) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA725107, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Growth Hormone in human serum samples. Capture: HA725106, Human Growth Hormone Rabbit mAb [PSH12-81] Detector: HA725107, Human Growth Hormone Rabbit mAb [PSH12-82] Interpolated concentration of native Growth Hormone was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 1522 pg/mL in Human serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-82)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human Growth Hormone matched pair antibodies Capture: HA725106, Human Growth Hormone Rabbit mAb [PSH12-81] Detector: HA725107, Human Growth Hormone Rabbit mAb [PSH12-82] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725106) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Growth Hormone protein (HA211027) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA725107, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Growth Hormone in human serum samples. Capture: HA725106, Human Growth Hormone Rabbit mAb [PSH12-81] Detector: HA725107, Human Growth Hormone Rabbit mAb [PSH12-82] Interpolated concentration of native Growth Hormone was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 1522 pg/mL in Human serum.
quantity: 100μl
price: 409.00 USD
to the supplier