Rat IFN gamma
HUABIO
catalog: HA721618

domestic rabbit monoclonal (PS01-96)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of Rat IFN gamma matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721618) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IFN gamma protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721619) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Rat IFN gamma
HUABIO
catalog: HA721619

domestic rabbit monoclonal (PS01-97)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of Rat IFN gamma matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721618) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IFN gamma protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721619) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Rat IFN gamma
HUABIO
catalog: HA721620

domestic rabbit monoclonal (PS01-97)
reactivity: rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Rat IFN gamma matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721618) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rat IFN gamma protein starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721620) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Rat IFN gamma
HUABIO
catalog: HA722421

domestic rabbit monoclonal (PSH05-69)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of rat IFNG matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722421) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Rat IFN gamma protein (HA210577) starting from 2,500 pg/ml to 0 pg/ml and detect antibody (HA722422, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IFNG in Concanavalin A treated rat spleen(18 hours) supernatant samples. Rat spleen was cultured for 18 hours in the presence or absence of 3 µg/ml Concanavalin A. The concentrations of IFNG measured in duplicate and interpolated from the IFNG standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 6% and stimulated 6%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFNG concentration was determined to be 10.8 ng/ml in Concanavalin A stimulated rat spleen supernatant and undetectable in unstimulated rat spleen supernatant.

Interpolated concentrations of spiked IFNG in rat cell culture media samples. The concentrations of IFNG were interpolated from the IFNG standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.
quantity: 100μl
price: 649.00 USD
to the supplier

Rat IFN gamma
HUABIO
catalog: HA722422

domestic rabbit monoclonal (PSH05-70)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of rat IFNG matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722421) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Rat IFN gamma protein (HA210577) starting from 2,500 pg/ml to 0 pg/ml and detect antibody (HA722422, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IFNG in Concanavalin A treated rat spleen(18 hours) supernatant samples. Rat spleen was cultured for 18 hours in the presence or absence of 3 µg/ml Concanavalin A. The concentrations of IFNG measured in duplicate and interpolated from the IFNG standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 6% and stimulated 6%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFNG concentration was determined to be 10.8 ng/ml in Concanavalin A stimulated rat spleen supernatant and undetectable in unstimulated rat spleen supernatant.

Interpolated concentrations of spiked IFNG in rat cell culture media samples. The concentrations of IFNG were interpolated from the IFNG standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%.
quantity: 100μl
price: 649.00 USD
to the supplier

Rat IFN gamma
HUABIO
catalog: HA722589B

domestic rabbit monoclonal (PSH05-70)
reactivity: rat
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier