domestic rabbit monoclonal (SY25-01)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Smad3 on different lysates with Rabbit anti-Smad3 antibody (ET1607-41) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HL-60 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: bEnd.3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 1 minute 52 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-41) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of Smad3 with anti-Smad3 antibody (ET1607-41) at 1:1,000 dilution. Lane 1: Wild-type HaCaT whole cell lysate (15 µg). Lane 2: Smad3 knockout HaCaT whole cell lysate (15 µg). ET1607-41 was shown to specifically react with Smad3 in wild-type HaCaT cells. NO band was observed when Smad3 knockout sample was tested. Wild-type and Smad3 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-41, 1:1,000) was used in 5% BSA at room temperature for 2 hours. Goat anti-Rabbit IgG-HRP antibody at 1:10,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A549 cells labeling Smad3 with Rabbit anti-Smad3 antibody (ET1607-41) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Smad3 antibody (ET1607-41) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (SY25-01)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Smad3 on different lysates with Rabbit anti-Smad3 antibody (HA750123) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HL-60 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: bEnd.3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 1 minute 52 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750123) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of Smad3 with anti-Smad3 antibody (HA750123) at 1:1,000 dilution. Lane 1: Wild-type HaCaT whole cell lysate (15 µg). Lane 2: Smad3 knockout HaCaT whole cell lysate (15 µg). ET1607-41 was shown to specifically react with Smad3 in wild-type HaCaT cells. NO band was observed when Smad3 knockout sample was tested. Wild-type and Smad3 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-41, 1:1,000) was used in 5% BSA at room temperature for 2 hours. Goat anti-Rabbit IgG-HRP antibody at 1:10,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A549 cells labeling Smad3 with Rabbit anti-Smad3 antibody (HA750123) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Smad3 antibody (HA750123) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Immunocytochemical staining of Hela cells using anti-SMAD3 rabbit polyclonal antibody.
Immunocytochemical staining of HCT116 cells using anti-SMAD3 rabbit polyclonal antibody.
Immunocytochemical staining of HepG2 cells using anti-SMAD3 rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (SY25-01)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section