PCSK9
HUABIO
catalog: EM1701-26-50UL
mouse monoclonal (2F1)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of PCSK9 on different lysates with Mouse anti-PCSK9 antibody (EM1701-26) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: K-562 cell lysate Lane 3: SH-SY5Y cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 62,78 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HepG2 cells labeling PCSK9 with Mouse anti-PCSK9 antibody (EM1701-26) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PCSK9 antibody (EM1701-26) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
quantity: 50μl
price: 205.00 USD
to the supplier
PCSK9
HUABIO
catalog: HA722528
domestic rabbit monoclonal (JE30-37)
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA722528) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (negative) Lane 2: Huh7 cell lysate Lane 3: BxPC-3 cell lysate Lane 4: HeLa cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 60/74 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722528) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PCSK9 antibody (HA722528) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PCSK9 antibody (HA722528) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Human PCSK9
HUABIO
catalog: HA725124
domestic rabbit monoclonal (PSH12-85)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human PCSK9 matched pair antibodies Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725124) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human PCSK9 protein (HA210991) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725125, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract. Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] The concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 50% and HepG2 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 9,074 pg/ml in HepG2 cell culture supernatant and 46,405 pg/ml in HepG2 cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human PCSK9
HUABIO
catalog: HA725125
domestic rabbit monoclonal (PSH12-86)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human PCSK9 matched pair antibodies Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725124) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human PCSK9 protein (HA210991) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725125, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract. Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] The concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 50% and HepG2 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 9,074 pg/ml in HepG2 cell culture supernatant and 46,405 pg/ml in HepG2 cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human PCSK9
HUABIO
catalog: HA725126B
domestic rabbit monoclonal (PSH12-86)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human PCSK9 matched pair antibodies Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725124) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human PCSK9 protein (HA210991) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725125, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract. Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] The concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 50% and HepG2 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PCSK9 concentration was determined to be 9,074 pg/ml in HepG2 cell culture supernatant and 46,405 pg/ml in HepG2 cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier
Human PCSK9
HUABIO
catalog: HA725126H
domestic rabbit monoclonal (PSH12-86)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human PCSK9 matched pair antibodies Capture: HA725124, Human PCSK9 Rabbit mAb [PSH12-85] Detector: HA725125, Human PCSK9 Rabbit mAb [PSH12-86] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725124) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human PCSK9 protein (HA210991) starting from 2,500,000 pg/ml to 0 pg/ml and detect antibody (HA725125, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
PCSK9
HUABIO
catalog: EM1701-26
mouse monoclonal (2F1)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
quantity: 100μl
price: 360.00 USD
to the supplier