ICAM1
HUABIO
catalog: ER1910-98
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (ER1910-98) at 1/5,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat kidney tissue lysate Lane 3: Rat spleen tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 60 kDa Observed band size: 75-120 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1910-98) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (ER1910-98) at 1/10,000 dilution. Lane 1: C6 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60-120 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1910-98) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of RAW264.7 cells labeling ICAM1 with Rabbit anti-ICAM1 antibody (ER1910-98) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ICAM1 antibody (ER1910-98) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier
ICAM1
HUABIO
catalog: HA723878
domestic rabbit monoclonal (PSH16-76)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723878) at 1/10,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: Mouse kidney tissue lysate (30 µg/Lane) Lane 3: Mouse spleen tissue lysate (30 µg/Lane) Lane 4: Mouse liver tissue lysate (30 µg/Lane) Lane 5: Rat spleen tissue lysate (30 µg/Lane) Lane 6: Rat kidney tissue lysate (30 µg/Lane) Predicted band size: 60 kDa Observed band size: 75-100 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723878) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA723878) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60-120 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723878) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ICAM1 antibody (HA723878) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723878) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Rat ICAM1
HUABIO
catalog: HA723926
domestic rabbit monoclonal (PSH17-51)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ICAM1 in rat serum samples. Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 62,341 pg/ml in rat serum.

Interpolated concentrations of spiked ICAM1 in rat cell culture media samples. Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Rat ICAM1
HUABIO
catalog: HA723927
domestic rabbit monoclonal (PSH17-52)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723928) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ICAM1 in rat serum samples. Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 62,341 pg/ml in rat serum.
quantity: 100μl
price: 649.00 USD
to the supplier
Rat ICAM1
HUABIO
catalog: HA723927B
domestic rabbit monoclonal (PSH17-52)
reactivity: rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723928) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ICAM1 in rat serum samples. Capture: HA723926, Rat ICAM1 Rabbit mAb [PSH17-51] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 62,341 pg/ml in rat serum.
quantity: 100μl
price: 385.00 USD
to the supplier
Rat ICAM1
HUABIO
catalog: HA723928
domestic rabbit monoclonal (PSH17-53)
reactivity: rat
application: ELISA

Sandwich ELISA analysis of Rat ICAM1 matched pair antibodies Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723928) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Rat ICAM1 protein (HA211063) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native ICAM1 in rat serum samples. Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 21,617 pg/ml in rat serum.

Interpolated concentrations of spiked ICAM1 in rat cell culture media samples. Capture: HA723928, Rat ICAM1 Rabbit mAb [PSH17-53] Detector: HA723927, Rat ICAM1 Rabbit mAb [PSH17-52] The concentrations of ICAM1 were measured in duplicates, interpolated from the ICAM1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
ICAM1
HUABIO
catalog: HA751605
domestic rabbit monoclonal (PSH16-76)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA751605) at 1/10,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: Mouse kidney tissue lysate (30 µg/Lane) Lane 3: Mouse spleen tissue lysate (30 µg/Lane) Lane 4: Mouse liver tissue lysate (30 µg/Lane) Lane 5: Rat spleen tissue lysate (30 µg/Lane) Lane 6: Rat kidney tissue lysate (30 µg/Lane) Predicted band size: 60 kDa Observed band size: 75-100 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751605) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody (HA751605) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60-120 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751605) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ICAM1 antibody (HA751605) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751605) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier