domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Osteopontin on different lysates with Rabbit anti-Osteopontin antibody (0806-6) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours, add 1μg/mL BFA for last 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 60 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0806-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH09-23)
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Osteopontin antibody (HA723081) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunofluorescence analysis of paraffin-embedded human bone tissue labeling Osteopontin with Rabbit anti-Osteopontin antibody (HA723081) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) overnight at 60℃ water bath. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723081) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-11)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Immunohistochemical analysis of paraffin-embedded mouse knee joint tissue with Rabbit anti-Osteopontin antibody (HA723601) at 1/9,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723601) at 1/9,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH09-23)
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Osteopontin antibody (HA751277) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751277) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunofluorescence analysis of paraffin-embedded human bone tissue labeling Osteopontin with Rabbit anti-Osteopontin antibody (HA751277) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) overnight at 60℃ water bath. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751277) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-11)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Immunohistochemical analysis of paraffin-embedded mouse knee joint tissue with Rabbit anti-Osteopontin antibody (HA751506) at 1/9,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751506) at 1/9,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: kidney Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier