RICTOR
HUABIO
catalog: HA721141

domestic rabbit monoclonal (1D5)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of RICTOR on different lysates with Rabbit anti-RICTOR antibody (HA721141) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: SW480 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 192 kDa Observed band size: 192 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721141) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A431 cells labeling RICTOR with Rabbit anti-RICTOR antibody (HA721141) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RICTOR antibody (HA721141) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow cytometric analysis of Hela cells labeling RICTOR. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721141, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

RICTOR
HUABIO
catalog: HA721142

domestic rabbit monoclonal (1A5)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of RICTOR on HepG2 cell lysates with Rabbit anti-RICTOR antibody (HA721142) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 192 kDa Observed band size: 192 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721142) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-RICTOR antibody (HA721142) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721142) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry analysis of A431 cells labeling RICTOR with Rabbit anti-RICTOR antibody (HA721142) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RICTOR antibody (HA721142) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-RICTOR (T1135)
HUABIO
catalog: HA723161

domestic rabbit monoclonal (PSH09-92)
reactivity: human, mouse
application: western blot

Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA723161) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate Lane 3: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 192 kDa Observed band size: 240 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723161) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA723161) at 1/2,000 dilution. Lane 1: C2C12 starved overnight cell lysate Lane 2: C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 192 kDa Observed band size: 240 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723161) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

RICTOR
HUABIO
catalog: HA750483

domestic rabbit monoclonal (1D5)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of RICTOR on different lysates with Rabbit anti-RICTOR antibody (HA750483) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: SW480 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 192 kDa Observed band size: 192 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750483) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A431 cells labeling RICTOR with Rabbit anti-RICTOR antibody (HA750483) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RICTOR antibody (HA750483) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow cytometric analysis of Hela cells labeling RICTOR. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750483, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier

RICTOR
HUABIO
catalog: HA750484

domestic rabbit monoclonal (1A5)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of RICTOR on HepG2 cell lysates with Rabbit anti-RICTOR antibody (HA750484) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 192 kDa Observed band size: 192 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750484) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-RICTOR antibody (HA750484) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750484) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry analysis of A431 cells labeling RICTOR with Rabbit anti-RICTOR antibody (HA750484) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RICTOR antibody (HA750484) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-RICTOR (T1135)
HUABIO
catalog: HA751323

domestic rabbit monoclonal (PSH09-92)
reactivity: human, mouse
application: western blot

Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA751323) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate Lane 3: HepG2 treated with 100nM Calyculin A for 30 minutes then add 100nM insulin for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 192 kDa Observed band size: 240 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751323) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-RICTOR (T1135) on different lysates with Rabbit anti-Phospho-RICTOR (T1135) antibody (HA751323) at 1/2,000 dilution. Lane 1: C2C12 starved overnight cell lysate Lane 2: C2C12 starved overnight then treated with 100nM Calyculin A for 30 minutes then add 100ng/mL insulin for 20 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 192 kDa Observed band size: 240 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751323) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier