HUABIO Cycs antibody

Cytochrome C
HUABIO
catalog: ET1610-16

domestic rabbit monoclonal (SC59-01)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (ET1610-16) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: C6 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (ET1610-16) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-16) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytochrome C antibody (ET1610-16) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Cytochrome C
HUABIO
catalog: ET1610-60

domestic rabbit monoclonal (SC69-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (ET1610-60) at 1/2,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: C6 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-60) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (ET1610-60) at 1/2,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: Human kidney tissue lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-60) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of C6 cells labeling Cytochrome C with Rabbit anti-Cytochrome C antibody (ET1610-60) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytochrome C antibody (ET1610-60) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Cytochrome C
HUABIO
catalog: HA750202

domestic rabbit monoclonal (SC59-01)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (HA750202) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: C6 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Rat brain tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750202) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (HA750202) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750202) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytochrome C antibody (HA750202) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750202) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Cytochrome C
HUABIO
catalog: HA750226

domestic rabbit monoclonal (SC69-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section