domestic rabbit monoclonal (PSH14-02)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Phospho-BCKDHA (S293) on different lysates with Rabbit anti-Phospho-BCKDHA (S293) antibody (HA723592) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: NIH/3T3 cell lysate (10 µg/Lane) Lane 4: C6 cell lysate (10 µg/Lane) Lane 5: COS-1 cell lysate (10 µg/Lane) Lane 6: Mouse liver tissue lysate (20 µg/Lane) Lane 7: Rat liver tissue lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour (10 µg/Lane) Predicted band size: 50 kDa Observed band size: 48 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723592) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-62)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of BCKDHA on different lysates with Rabbit anti-BCKDHA antibody (HA723769) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A20 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 48 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723769) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling BCKDHA with Rabbit anti-BCKDHA antibody (HA723769) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCKDHA antibody (HA723769) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of NIH/3T3 cells labeling BCKDHA with Rabbit anti-BCKDHA antibody (HA723769) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCKDHA antibody (HA723769) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-02)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Phospho-BCKDHA (S293) on different lysates with Rabbit anti-Phospho-BCKDHA (S293) antibody (HA751498) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: NIH/3T3 cell lysate (10 µg/Lane) Lane 4: C6 cell lysate (10 µg/Lane) Lane 5: COS-1 cell lysate (10 µg/Lane) Lane 6: Mouse liver tissue lysate (20 µg/Lane) Lane 7: Rat liver tissue lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour (10 µg/Lane) Predicted band size: 50 kDa Observed band size: 48 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751498) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-62)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of BCKDHA on different lysates with Rabbit anti-BCKDHA antibody (HA751573) at 1/20,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A20 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 48 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751573) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling BCKDHA with Rabbit anti-BCKDHA antibody (HA751573) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCKDHA antibody (HA751573) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of NIH/3T3 cells labeling BCKDHA with Rabbit anti-BCKDHA antibody (HA751573) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BCKDHA antibody (HA751573) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier