HUABIO Stat1 antibody

STAT1 alpha/beta
HUABIO
catalog: ER31221

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of STAT1 alpha/beta on different lysates with Rabbit anti-STAT1 alpha/beta antibody (ER31221) at 1/20,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: SK-Br-3 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: L6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 87/83 kDa Observed band size: 87/83 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31221) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

ICC staining Stat-1α/β in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining Stat-1α/β in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier

Phospho-STAT1 (S727)
HUABIO
catalog: ET1611-20

domestic rabbit monoclonal (SN67-04)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Phospho-STAT1 (S727) on different lysates with Rabbit anti-Phospho-STAT1 (S727) antibody (ET1611-20) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Mouse brain treated with λpp for 1 hour tissue lysate (20 µg/Lane) Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-20) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells treated with or without λpp labeling Phospho-STAT1 (S727) with Rabbit anti-Phospho-STAT1 (S727) antibody (ET1611-20) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-STAT1 (S727) antibody (ET1611-20) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human breast invasive ductal tumor tissue with Rabbit anti-Phospho-STAT1 (S727) antibody (ET1611-20) at 1/200 dilution. A: Untreated human breast invasive ductal tumor tissue B: λ-PPase treated human breast invasive ductal tumor tissue C: Negative control The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-20) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

STAT1
HUABIO
catalog: ET1612-22

domestic rabbit monoclonal (SD20-75)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of STAT1 on different lysates with Mouse anti-STAT1 antibody (ET1612-22) at 1/5,000 dilution. Lane 1: PATU8988-parental cell lysate (10 µg/Lane) Lane 2: PATU8988-STAT1 KD cell lysate (10 µg/Lane) Predicted band size: 87/83 kDa Observed band size: 83 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-22)) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

ICC staining of STAT1 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-STAT1 (S727)
HUABIO
catalog: HA750247

domestic rabbit monoclonal (SN67-04)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Phospho-STAT1 (S727) on different lysates with Rabbit anti-Phospho-STAT1 (S727) antibody (HA750247) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Mouse brain treated with λpp for 1 hour tissue lysate (20 µg/Lane) Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750247) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells treated with or without λpp labeling Phospho-STAT1 (S727) with Rabbit anti-Phospho-STAT1 (S727) antibody (HA750247) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-STAT1 (S727) antibody (HA750247) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human breast invasive ductal tumor tissue with Rabbit anti-Phospho-STAT1 (S727) antibody (HA750247) at 1/200 dilution. A: Untreated human breast invasive ductal tumor tissue B: λ-PPase treated human breast invasive ductal tumor tissue C: Negative control The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750247) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

STAT1
HUABIO
catalog: R1408-2

domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot

Western blot analysis on cell lysates using anti- STAT1 rabbit polyclonal antibodies.

ICC staining STAT1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier