domestic rabbit polyclonal
reactivity: human
application: western blot
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Placental alkaline phosphatase
HUABIO
catalog: ET1705-21
domestic rabbit monoclonal (JM22-53)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Placental alkaline phosphatase on different lysates with Rabbit anti-Placental alkaline phosphatase antibody (ET1705-21) at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lane 3: AGS cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-21) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Placental alkaline phosphatase on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase antibody (ET1705-21) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-21) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Placental alkaline phosphatase antibody (ET1705-21) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-21) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
intestinal alkaline phosphatase
HUABIO
catalog: HA723952
domestic rabbit monoclonal (PSH17-83)
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA723952) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723952) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA723952) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723952) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA723952) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723952) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Alkaline Phosphatase
HUABIO
catalog: M0806-10
mouse monoclonal (6-10)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section