Mouse IL-27A p28
HUABIO
catalog: HA723624

domestic rabbit monoclonal (PSH14-31)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-27A p28 matched pair antibodies Capture: HA723624, Mouse IL-27A p28 Rabbit mAb [PSH14-31] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723624) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-27A p28 protein (HA211005) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723625, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-27A p28 in LPS stimulated RAW 264.7 cell culture supernatant. Capture: HA723624, Mouse IL-27A p28 Rabbit mAb [PSH14-31] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] RAW 264.7 cells were cultured for 48 hours and stimulated with 5 µg/ml LPS. The concentrations of IL-27A p28 were measured in duplicates, interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are stimulated RAW 264.7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-27A p28 concentration was to be 2,074 pg/ml in LPS stimulated RAW 264.7 cell culture supernatant.

Interpolated concentrations of native IL-27A p28 in RAW 264.7 cell culture supernatant. Capture: HA723624, Mouse IL-27A p28 Rabbit mAb [PSH14-31] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] RAW2 64.7 were stimulated with 5 µg/ml LPS and incubated for 48 hours. The concentrations of IL-27A p28 were interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are LPS stimulated RAW 264.7 cell culture supernatant 50% and unstimulated RAW 264.7 cell culture supernatant 50%. The mean IL-27A p28 concentration was determined to be 2,074 pg/ml in LPS stimulated RAW 264.7 cell culture supernatant and undetectable in the unstimulated RAW 264.7 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-27A p28
HUABIO
catalog: HA723625

domestic rabbit monoclonal (PSH14-32)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-27A p28 matched pair antibodies Capture: HA723624, Mouse IL-27A p28 Rabbit mAb [PSH14-31] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723624) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-27A p28 protein (HA211005) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723625, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse IL-27A p28 matched pair antibodies Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723627) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-27A p28 protein (HA211005) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723625, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-27A p28 in LPS stimulated RAW 264.7 cell culture supernatant. Capture: HA723624, Mouse IL-27A p28 Rabbit mAb [PSH14-31] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] RAW 264.7 cells were cultured for 48 hours and stimulated with 5 µg/ml LPS. The concentrations of IL-27A p28 were measured in duplicates, interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are stimulated RAW 264.7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-27A p28 concentration was to be 2,074 pg/ml in LPS stimulated RAW 264.7 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-27A p28
HUABIO
catalog: HA723626B

domestic rabbit monoclonal (PSH14-32)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse IL-27A p28 matched pair antibodies Capture: HA723624, Mouse IL-27A p28 Rabbit mAb [PSH14-31] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723624) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-27A p28 protein (HA211005) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723625, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse IL-27A p28 matched pair antibodies Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723627) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-27A p28 protein (HA211005) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723625, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-27A p28 in LPS stimulated RAW 264.7 cell culture supernatant. Capture: HA723624, Mouse IL-27A p28 Rabbit mAb [PSH14-31] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] RAW 264.7 cells were cultured for 48 hours and stimulated with 5 µg/ml LPS. The concentrations of IL-27A p28 were measured in duplicates, interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are stimulated RAW 264.7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-27A p28 concentration was to be 2,074 pg/ml in LPS stimulated RAW 264.7 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-27A p28
HUABIO
catalog: HA723627

domestic rabbit monoclonal (PSH14-33)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-27A p28 matched pair antibodies Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723627) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-27A p28 protein (HA211005) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723625, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-27A p28 in LPS stimulated RAW 264.7 cell culture supernatant. Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] RAW 264.7 cells were cultured for 48 hours and stimulated with 5 µg/ml LPS. The concentrations of IL-27A p28 were measured in duplicates, interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are stimulated RAW 264.7 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-27A p28 concentration was to be 1,264 pg/ml in LPS stimulated RAW 264.7 cell culture supernatant.

Interpolated concentrations of native IL-27A p28 in RAW 264.7 cell culture supernatant. Capture: HA723627, Mouse IL-27A p28 Rabbit mAb [PSH14-33] Detector: HA723625, Mouse IL-27A p28 Rabbit mAb [PSH14-32] RAW2 64.7 were stimulated with 5 µg/ml LPS and incubated for 48 hours. The concentrations of IL-27A p28 were interpolated from the IL-27A p28 standard curves and corrected for sample dilution. Undiluted samples are LPS stimulated RAW 264.7 cell culture supernatant 50% and unstimulated RAW 264.7 cell culture supernatant 50%. The mean IL-27A p28 concentration was determined to be 1,264 pg/ml in LPS stimulated RAW 264.7 cell culture supernatant and undetectable in the unstimulated RAW 264.7 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

IL-27A p28
HUABIO
catalog: HA723695

domestic rabbit monoclonal (PSH14-93)
reactivity: mouse
application: western blot

Western blot analysis of IL-27A p28 on different lysates with Rabbit anti-IL-27A p28 antibody (HA723695) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723695) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

IL-27A p28
HUABIO
catalog: HA751537

domestic rabbit monoclonal (PSH14-93)
reactivity: mouse
application: western blot

Western blot analysis of IL-27A p28 on different lysates with Rabbit anti-IL-27A p28 antibody (HA751537) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then treated with 100ng/mL LPS for 4 hours add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751537) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier