Human IL-27 p28
HUABIO
catalog: HA722044
domestic rabbit monoclonal (PSH03-80)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human IL-27 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722044 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-27 protein starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722045-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-27 p28
HUABIO
catalog: HA722045
domestic rabbit monoclonal (PSH03-81)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human IL-27 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722044 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-27 protein starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722045-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-27
HUABIO
catalog: HA722046
domestic rabbit monoclonal (PSH03-82)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human IL-27 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722046 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-27 protein starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722047-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-27
HUABIO
catalog: HA722047
domestic rabbit monoclonal (PSH03-83)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human IL-27 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722046 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-27 protein starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722047-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-27
HUABIO
catalog: HA722048
domestic rabbit monoclonal (PSH03-81)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human IL-27 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722048 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-27 protein starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722049-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-27
HUABIO
catalog: HA722049
domestic rabbit monoclonal (PSH03-82)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human IL-27 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722048 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-27 protein starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722049-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-27 p28
HUABIO
catalog: HA722620B
domestic rabbit monoclonal (PSH03-81)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier
Human IL-27
HUABIO
catalog: HA722621B
domestic rabbit monoclonal (PSH03-83)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier
Human IL-27
HUABIO
catalog: HA722622B
domestic rabbit monoclonal (PSH03-82)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier