HUABIO Pdgfrb antibody

PDGFR beta
HUABIO
catalog: ET1605-20

domestic rabbit monoclonal (SY10-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-PDGFR beta antibody (ET1605-20) at 1/200 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1605-20, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-PDGFR beta antibody (ET1605-20) at 1/200 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1605-20, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western blot analysis of PDGFR beta on different lysates with Rabbit anti-PDGFR beta antibody (ET1605-20) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-PDGFR beta (Y740)
HUABIO
catalog: ET1611-29

domestic rabbit monoclonal (SN0646)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-PDGFR beta (Y740) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y740) antibody (ET1611-29) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes whole cell lysate Lane 3: NIH/3T3 treated with λpp whole cell lysate Lane 4: NIH/3T3 treated with 50 ng/mL PDGF for 5 minutes then treated with λpp whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-29) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

ICC staining PDGF Receptor beta(phospho Y740) in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining PDGF Receptor beta(phospho Y740) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-PDGFR beta (Y1009)
HUABIO
catalog: HA721736

domestic rabbit monoclonal (JE46-72)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (HA721736) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa (Glycosylation) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721736) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (HA721736) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa (Glycosylation) Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721736) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-PDGFR beta (Y1021)
HUABIO
catalog: HA721833

domestic rabbit monoclonal (JE48-54)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-PDGFR beta (Y1021) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1021) antibody (HA721833) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 100 ng/mL PDGF for 5 minutes whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-PDGFR beta (Y751)
HUABIO
catalog: HA723550

domestic rabbit monoclonal (PSH13-61)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-PDGFR beta (Y751) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y751) antibody (HA723550) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate Lane 5: NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lane 6: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723550) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

PDGFR beta
HUABIO
catalog: HA750082

Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-PDGFR beta antibody (HA750082) at 1/200 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750082, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-PDGFR beta antibody (HA750082) at 1/200 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750082, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western blot analysis of PDGFR beta on different lysates with Rabbit anti-PDGFR beta antibody (HA750082) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750082) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-PDGFR beta (Y751)
HUABIO
catalog: HA751480

domestic rabbit monoclonal (PSH13-61)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-PDGFR beta (Y751) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y751) antibody (HA751480) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate Lane 5: NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lane 6: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751480) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

PDGFR beta
HUABIO
catalog: R1510-44

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

ICC staining PDGF Receptor beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining PDGF Receptor beta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining PDGF Receptor beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier