domestic rabbit monoclonal (JE75-16)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of PDGF B on different lysates with Rabbit anti-PDGF B antibody (HA722003) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722003) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of PDGF B on different lysates with Rabbit anti-PDGF B antibody (HA722003) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722003) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PDGF B antibody (HA722003) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722003) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-05)
reactivity: mouse, rat
application: ELISA
reactivity: mouse, rat
application: ELISA
Sandwich ELISA analysis of Mouse PDGF-BB matched pair antibodies Capture: HA723810, Mouse PDGF-BB Rabbit mAb [PSH16-05] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723810) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse PDGF-BB protein (HA211037) protein starting from 500 pg/ml to 0 pg/ml and detect antibody (HA723811, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native PDGF-BB in mouse and rat samples. Capture: HA723810, Mouse PDGF-BB Rabbit mAb [PSH16-05] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Interpolated concentration of native PDGF-BB was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PDGF-BB concentration was determined to be 32,761 pg/mL in mouse Serum and 8,427 pg/ml in rat serum. There was no detectable signal in C2C12 cell culture supernatant.
Interpolated concentrations of spiked PDGF-BB in cell culture media samples. Capture: HA723810, Mouse PDGF-BB Rabbit mAb [PSH16-05] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] The concentrations of PDGF-BB were measured in duplicates, interpolated from the PDGF-BB standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-06)
reactivity: mouse, rat
application: ELISA
reactivity: mouse, rat
application: ELISA
Sandwich ELISA analysis of Mouse PDGF-BB matched pair antibodies Capture: HA723810, Mouse PDGF-BB Rabbit mAb [PSH16-05] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723810) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse PDGF-BB protein (HA211037) protein starting from 500 pg/ml to 0 pg/ml and detect antibody (HA723811, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Mouse PDGF-BB matched pair antibodies Capture: HA723812, Mouse PDGF-BB Rabbit mAb [PSH16-07] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723812) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse PDGF-BB protein (HA211037) protein starting from 500 pg/ml to 0 pg/ml and detect antibody (HA723811, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native PDGF-BB in mouse and rat samples. Capture: HA723810, Mouse PDGF-BB Rabbit mAb [PSH16-05] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Interpolated concentration of native PDGF-BB was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PDGF-BB concentration was determined to be 32,761 pg/mL in mouse Serum and 8,427 pg/ml in rat serum. There was no detectable signal in C2C12 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-06)
reactivity: mouse, rat
conjugate: biotin
application: ELISA
reactivity: mouse, rat
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse PDGF-BB matched pair antibodies Capture: HA723810, Mouse PDGF-BB Rabbit mAb [PSH16-05] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723810) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse PDGF-BB protein (HA211037) protein starting from 500 pg/ml to 0 pg/ml and detect antibody (HA723811, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Mouse PDGF-BB matched pair antibodies Capture: HA723812, Mouse PDGF-BB Rabbit mAb [PSH16-07] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723812) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse PDGF-BB protein (HA211037) protein starting from 500 pg/ml to 0 pg/ml and detect antibody (HA723811, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native PDGF-BB in mouse and rat samples. Capture: HA723810, Mouse PDGF-BB Rabbit mAb [PSH16-05] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Interpolated concentration of native PDGF-BB was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PDGF-BB concentration was determined to be 32,761 pg/mL in mouse Serum and 8,427 pg/ml in rat serum. There was no detectable signal in C2C12 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-07)
reactivity: mouse, rat
application: ELISA
reactivity: mouse, rat
application: ELISA
Sandwich ELISA analysis of Mouse PDGF-BB matched pair antibodies Capture: HA723812, Mouse PDGF-BB Rabbit mAb [PSH16-07] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723812) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse PDGF-BB protein (HA211037) protein starting from 500 pg/ml to 0 pg/ml and detect antibody (HA723811, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native PDGF-BB in mouse samples. Capture: HA723812, Mouse PDGF-BB Rabbit mAb [PSH16-07] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] Interpolated concentration of native PDGF-BB was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean PDGF-BB concentration was determined to be 41,600 pg/mL in mouse Serum. There was no detectable signal in C2C12 cell culture supernatant.
Interpolated concentrations of spiked PDGF-BB in cell culture media samples. Capture: HA723812, Mouse PDGF-BB Rabbit mAb [PSH16-07] Detector: HA723811, Mouse PDGF-BB Rabbit mAb [PSH16-06] The concentrations of PDGF-BB were measured in duplicates, interpolated from the PDGF-BB standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier