HUABIO Gad2 antibody

GAD65
HUABIO
catalog: HA722750

domestic rabbit monoclonal (PSH06-80)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

GAD65
HUABIO
catalog: HA751102

domestic rabbit monoclonal (PSH06-80)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier

GAD65
HUABIO
catalog: HA601389

domestic rabbit polyclonal (PSH06-80)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 2,000 (GAD65, HA601389, green); 1:2,000 (EAAT1, ET1704-54, red) Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 2,000 (GAD65, HA601389, green); 1:2,000 (EAAT1, ET1704-54, red) Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-GAD65 antibody (HA601389) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier

GAD65
HUABIO
catalog: HA601455

domestic rabbit polyclonal (PSH06-80)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GAD65 antibody (HA601455) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601455) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

GAD65
HUABIO
catalog: HA601473

domestic rabbit polyclonal (PSH06-80)
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of GAD65 on different lysates with Chicken anti-GAD65 antibody (HA601473) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601473) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

GAD65
HUABIO
catalog: HA610232

domestic rabbit polyclonal (PSH06-80)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 2,000 (GAD65, HA610232, green); 1:2,000 (EAAT1, ET1704-54, red) Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 2,000 (GAD65, HA610232, green); 1:2,000 (EAAT1, ET1704-54, red) Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-GAD65 antibody (HA610232) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610232) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

GAD65
HUABIO
catalog: HA610289

domestic rabbit polyclonal (PSH06-80)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-GAD65 antibody (HA610289) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610289) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GAD65 antibody (HA610289) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610289) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

GAD65
HUABIO
catalog: HA610307

domestic rabbit polyclonal (PSH06-80)
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of GAD65 on different lysates with Chicken anti-GAD65 antibody (HA610307) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610307) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier