domestic rabbit monoclonal (SC06-28)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (ET1610-96) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: HEK-293 cell lysate (10 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: SH-SY5Y cell lysate (10 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-96) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (ET1610-96) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NSE KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-96) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (SC06-28)
reactivity: human, mouse, rat
application: flow cytometry
reactivity: human, mouse, rat
application: flow cytometry
Immunocytochemistry analysis of SH-SY5Y cells labeling NSE with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NSE antibody (HA720178F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Flow cytometric analysis of SH-SY5Y cells labeling NSE. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with NSE (HA720178F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 429.00 USD
to the supplier
domestic rabbit monoclonal (SC06-28)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (HA750239) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: HEK-293 cell lysate (10 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: SH-SY5Y cell lysate (10 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750239) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (HA750239) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NSE KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750239) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750239, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot
reactivity: human, mouse, rat, zebrafish
application: western blot
Western blot analysis of NSE on different lysates with Rabbit anti-NSE antibody (R1401-6) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NSE KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1401-6) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis on different cell lysates using anti-NSE rabbit polyclonal antibodies.
Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysate using anti-NSE antibody at 1/500 dilution.
quantity: 100μl
price: 330 USD
to the supplier