Complement C3
HUABIO
catalog: ET1702-99

domestic rabbit monoclonal (JF10-30)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (ET1702-99) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 187 kDa Observed band size: 108/37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-99) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HepG2 cells labeling Complement C3 with Rabbit anti-Complement C3 antibody (ET1702-99) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C3 antibody (ET1702-99) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HepG2 cells labeling Complement C3. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-99, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

Complement C3
HUABIO
catalog: HA723984

domestic rabbit monoclonal (PSH18-31)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (HA723984) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 186 kDa Observed band size: 43-186 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723984) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

Mouse/Rat Complement C3
HUABIO
catalog: HA725143

domestic rabbit monoclonal (PSH16-81)
reactivity: mouse, rat
application: ELISA

Sandwich ELISA analysis of Mouse Complement C3 matched pair antibodies Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725143) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement C3 protein (HA211093) protein starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA725144, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Complement C3 in mouse serum samples. Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] The concentrations of Complement C3 were measured in triplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.0005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Complement C3 concentration was determined to be 1,269 µg/ml in mouse serum.

Interpolated concentrations of native Complement C3 in rat serum samples. Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are rat serum 0.000313%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 3,772 µg/ml in rat serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse/Rat Complement C3
HUABIO
catalog: HA725144

domestic rabbit monoclonal (PSH16-82)
reactivity: mouse, rat
application: ELISA

Sandwich ELISA analysis of Mouse Complement C3 matched pair antibodies Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725143) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement C3 protein (HA211093) protein starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA725144, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Complement C3 in mouse serum samples. Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] The concentrations of Complement C3 were measured in triplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.0005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Complement C3 concentration was determined to be 1,269 µg/ml in mouse serum.

Interpolated concentrations of native Complement C3 in rat serum samples. Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are rat serum 0.000313%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 3,772 µg/ml in rat serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse/Rat Complement C3
HUABIO
catalog: HA725145B

domestic rabbit monoclonal (PSH16-82)
reactivity: mouse, rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse Complement C3 matched pair antibodies Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725143) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement C3 protein (HA211093) protein starting from 40,000 pg/ml to 0 pg/ml and detect antibody (HA725144, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Complement C3 in mouse serum samples. Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] The concentrations of Complement C3 were measured in triplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.0005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean Complement C3 concentration was determined to be 1,269 µg/ml in mouse serum.

Interpolated concentrations of native Complement C3 in rat serum samples. Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] The concentrations of Complement C3 were measured in duplicates, interpolated from the Complement C3 standard curve and corrected for sample dilution. Undiluted samples are rat serum 0.000313%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Complement C3 concentration was determined to be 3,772 µg/ml in rat serum.
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse/Rat Complement C3
HUABIO
catalog: HA725145H

domestic rabbit monoclonal (PSH16-82)
reactivity: mouse, rat
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Mouse Complement C3 matched pair antibodies Capture: HA725143, Mouse/Rat Complement C3 Rabbit mAb [PSH16-81] Detector: HA725144, Mouse/Rat Complement C3 Rabbit mAb [PSH16-82] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725143) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Complement C3 protein (HA211093) protein starting from 1,000,000 pg/ml to 0 pg/ml and detect antibody (HA725144, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Complement C3
HUABIO
catalog: HA750367

domestic rabbit monoclonal (JF10-30)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Complement C3 on different lysates with Rabbit anti-Complement C3 antibody (HA750367) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 187 kDa Observed band size: 108/37 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750367) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HepG2 cells labeling Complement C3 with Rabbit anti-Complement C3 antibody (HA750367) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C3 antibody (HA750367) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HepG2 cells labeling Complement C3. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750367, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier