FAM38A / PIEZO1
HUABIO
catalog: HA601100-50UL

mouse monoclonal (2-10-R-A)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of FAM38A / PIEZO1 on recombinant protein lysates with Mouse anti-FAM38A / PIEZO1 antibody (HA601100) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601100) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (HA601100) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601100) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-FAM38A / PIEZO1 antibody (HA601100) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601100) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier

FAM38A / PIEZO1
HUABIO
catalog: HA610024

mouse monoclonal (2-10-R-A)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of FAM38A / PIEZO1 on recombinant protein lysates with Mouse anti-FAM38A / PIEZO1 antibody (HA610024) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610024) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A / PIEZO1 antibody (HA610024) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610024) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-FAM38A / PIEZO1 antibody (HA610024) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610024) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

FAM38A / PIEZO1
HUABIO
catalog: HA723341

domestic rabbit monoclonal (PSH11-49)
reactivity: mouse
application: western blot

Western blot analysis of FAM38A / PIEZO1 on different lysates with Rabbit anti-FAM38A / PIEZO1 antibody (HA723341) at 1/2,000 dilution. Lane 1: 293T transfected with mPiezo1 cell lysate Lane 2: 293T transfected with mPiezo2 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes 50 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723341) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of 293T cells labeling FAM38A / PIEZO1 with Rabbit anti-FAM38A / PIEZO1 antibody (HA723341) at 1/200 dilution. 293T cells, transfected with GFP-tagged mouse Piezo1 (top, positive) / tag-free mouse Piezo2 (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38A / PIEZO1 antibody (HA723341) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier

FAM38A / PIEZO1
HUABIO
catalog: HA751411

domestic rabbit monoclonal (PSH11-49)
reactivity: mouse
application: western blot

Western blot analysis of FAM38A / PIEZO1 on different lysates with Rabbit anti-FAM38A / PIEZO1 antibody (HA751411) at 1/2,000 dilution. Lane 1: 293T transfected with mPiezo1 cell lysate Lane 2: 293T transfected with mPiezo2 cell lysate (negative) Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes 50 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of 293T cells labeling FAM38A / PIEZO1 with Rabbit anti-FAM38A / PIEZO1 antibody (HA751411) at 1/200 dilution. 293T cells, transfected with GFP-tagged mouse Piezo1 (top, positive) / tag-free mouse Piezo2 (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38A / PIEZO1 antibody (HA751411) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 649.00 USD
to the supplier

FAM38A / PIEZO1
HUABIO
catalog: M1005-2-50UL

mouse monoclonal (2-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of FAM38A / PIEZO1 on recombinant protein with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of FAM38A / PIEZO1 on MCF-7 cell lysates with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 287 kDa Observed band size: 287 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of Hela cells labeling FAM38A / PIEZO1 with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A / PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 50μl
price: 205.00 USD
to the supplier

FAM38A / PIEZO1
HUABIO
catalog: M1005-2

mouse monoclonal (2-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

quantity: 100μl
price: 360.00 USD
to the supplier

Anti-FAM38A/PIEZO1 Antibody [2-10]
HUABIO
catalog: M1005-2TR

mouse monoclonal (2-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

quantity: 20 uL
price: 99 USD
to the supplier