PSMA
HUABIO
catalog: ER1802-10
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of PSMA on different lysates with Rabbit anti-PSMA antibody (ER1802-10) at 1/1,000 dilution. Lane 1: LNCaP cell lysate (20 µg/Lane) Lane 2: PC-3 cell lysate (negative) (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 84 kDa Observed band size: 110 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
PSMA
HUABIO
catalog: HA600045
mouse monoclonal (A5G5)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of PSMA on different lysates with Mouse anti-PSMA antibody (HA600045) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600045) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PSMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600045, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-PSMA antibody (HA600045) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600045) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
PSMA
HUABIO
catalog: HA600046
mouse monoclonal (A5F12)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of PSMA on different lysates with Mouse anti-PSMA antibody (HA600046) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600046) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-PSMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600046, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-PSMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA600046, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
PSMA
HUABIO
catalog: HA601163
mouse monoclonal (A9H10)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of PSMA on different lysates with Mouse anti-PSMA antibody (HA601163) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of LNCaP (positive) and PC-3M (negative) cells labeling PSMA with Mouse anti-PSMA antibody (HA601163) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PSMA antibody (HA601163) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-PSMA antibody (HA601163) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601163) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
PSMA
HUABIO
catalog: HA601164
mouse monoclonal (A9H9)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of PSMA on different lysates with Mouse anti-PSMA antibody (HA601164) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601164) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of LNCaP (positive) and PC-3M (negative) cells labeling PSMA with Mouse anti-PSMA antibody (HA601164) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PSMA antibody (HA601164) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-PSMA antibody (HA601164) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601164) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
PSMA
HUABIO
catalog: HA601169
mouse monoclonal (A10A2)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of PSMA on different lysates with Mouse anti-PSMA antibody (HA601169) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601169) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of PSMA on different lysates with Mouse anti-PSMA antibody (HA601169) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-PSMA KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 84 kDa Observed band size: 110 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601169) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of LNCaP (positive) and PC-3M (negative) cells labeling PSMA with Mouse anti-PSMA antibody (HA601169) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PSMA antibody (HA601169) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 360.00 USD
to the supplier
PSMA
HUABIO
catalog: HA721593
domestic rabbit monoclonal (JE42-45)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of PSMA on different lysates with Rabbit anti-PSMA antibody (HA721593) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721593) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-PSMA antibody (HA721593) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721593) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
PSMA
HUABIO
catalog: HA721721
domestic rabbit monoclonal (PD00-72)
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of PSMA on different lysates with Rabbit anti-PSMA antibody (HA721721) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: 22RV1 cell lysate Lane 3: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 100/200 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721721) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of LNCaP (positive) and PC-3M (negative) cells labeling PSMA with Rabbit anti-PSMA antibody (HA721721) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PSMA antibody (HA721721) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-PSMA antibody (HA721721) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721721) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier