domestic rabbit monoclonal (JM81-10)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of FGFR3 on different lysates with Rabbit anti-FGFR3 antibody (ET1703-36) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: LNCaP cell lysate (15 µg/Lane) Lane 4: A549 cell lysate (15 µg/Lane) Predicted band size: 88 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-36) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FGFR3 on different lysates with Rabbit anti-FGFR3 antibody (ET1703-36) at 1/1,000 dilution. Lane 1: HEK293-si NT cell lysate Lane 2: HEK293-si FGFR3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ET1703-36 was shown to specifically react with FGFR3 in HEK293-si NT cells. Weakened band was observed when HEK293-si FGFR3 sample was tested. HEK293-si NT and HEK293-si FGFR3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-36, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling FGFR3 with Rabbit anti-FGFR3 antibody (ET1703-36) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FGFR3 antibody (ET1703-36) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (JM110-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of FGFR3 on different lysates with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: MCF-7 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FGFR3 on Rat skin tissue lysates with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 8 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-65) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:10,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FGFR3 on Mouse hippocampus lysates with Rabbit anti-FGFR3 antibody (ET1703-65) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-65) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier
domestic rabbit monoclonal (JM81-10)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of FGFR3 on different lysates with Rabbit anti-FGFR3 antibody (HA750379) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: LNCaP cell lysate (15 µg/Lane) Lane 4: A549 cell lysate (15 µg/Lane) Predicted band size: 88 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750379) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FGFR3 on different lysates with Rabbit anti-FGFR3 antibody (HA750379) at 1/1,000 dilution. Lane 1: HEK293-si NT cell lysate Lane 2: HEK293-si FGFR3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ET1703-36 was shown to specifically react with FGFR3 in HEK293-si NT cells. Weakened band was observed when HEK293-si FGFR3 sample was tested. HEK293-si NT and HEK293-si FGFR3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-36, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling FGFR3 with Rabbit anti-FGFR3 antibody (HA750379) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FGFR3 antibody (HA750379) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (JM110-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of FGFR3 on different lysates with Rabbit anti-FGFR3 antibody (HA750388) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: MCF-7 cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750388) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FGFR3 on Rat skin tissue lysates with Rabbit anti-FGFR3 antibody (HA750388) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 8 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750388) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:10,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FGFR3 on Mouse hippocampus lysates with Rabbit anti-FGFR3 antibody (HA750388) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 95 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750388) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (JM110-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section