domestic rabbit monoclonal (JJ09-34)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (ET1701-87) at 1/1,000 dilution. Lane 1: bEnd.3 cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse lung tissue lysate Lane 4: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: 5 minutes; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-87) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-45)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (negative) Lane 2: Human lung tissue lysate Lane 3: Rat lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: Lane 1-3 (left): 25 seconds; Lane 1-3 (right): 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-45)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (negative) Lane 2: Human lung tissue lysate Lane 3: Rat lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: Lane 1-3 (left): 25 seconds; Lane 1-3 (right): 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751166) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Von Willebrand Factor antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751166) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal
reactivity: mouse
reactivity: mouse
mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Von Willebrand Factor antibody (IRS244RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 278.00 USD
to the supplier