Mouse/Rat TNF alpha
HUABIO
catalog: HA721974

domestic rabbit monoclonal (PSH03-29)
reactivity: mouse, rat
application: ELISA

Sandwich ELISA analysis of rat TNF alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721974) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted rat TNF alpha protein (HA210538) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721975) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of mouse TNF alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721974) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse TNF alpha protein (HA210527) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721975)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Conditioned media was harvested after 72 hours. TNF-α was measured in 100% unstimulated and LPS stimulated rat spleen cell supernatant. The concentrations of TNF-α were interpolated from the TNF-α standard curves. The mean TNF-α concentration was determined to be 128 pg/mL in LPS stimulated rat spleen cell supernatant. There was no detectable signal in unstimulated supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse/Rat TNF alpha
HUABIO
catalog: HA721975

domestic rabbit monoclonal (PSH03-30)
reactivity: mouse, rat
application: ELISA

Sandwich ELISA analysis of rat TNF alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721974) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted rat TNF alpha protein (HA210538) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721975) -Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of mouse TNF alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721974) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted mouse TNF alpha protein (HA210527) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721975)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Conditioned media was harvested after 72 hours. TNF-α was measured in 100% unstimulated and LPS stimulated rat spleen cell supernatant. The concentrations of TNF-α were interpolated from the TNF-α standard curves. The mean TNF-α concentration was determined to be 128 pg/mL in LPS stimulated rat spleen cell supernatant. There was no detectable signal in unstimulated supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

TNF alpha
HUABIO
catalog: HA722022

domestic rabbit monoclonal (PS01-07)
reactivity: human, mouse
application: western blot

Western blot analysis of TNF alpha on different lysates with Rabbit anti-TNF alpha antibody (HA722022) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100ng/mL LPS for 7 hours, add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26/50/17 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Mouse TNF alpha
HUABIO
catalog: HA722040

domestic rabbit monoclonal (PSH03-76)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse TNF alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722040 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse TNF alpha protein starting from 1600 pg/ml to 0 pg/ml and detect antibody HA722041-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse TNF alpha
HUABIO
catalog: HA722041

domestic rabbit monoclonal (PSH03-77)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse TNF alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody HA722040 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse TNF alpha protein starting from 1600 pg/ml to 0 pg/ml and detect antibody HA722041-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse TNF alpha
HUABIO
catalog: HA722623B

domestic rabbit monoclonal (PSH03-77)
reactivity: mouse
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

Mouse/Rat TNF alpha
HUABIO
catalog: HA722626B

domestic rabbit monoclonal (PSH03-30)
reactivity: mouse, rat
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

TNF alpha
HUABIO
catalog: HA750897

domestic rabbit monoclonal (PS01-07)
reactivity: human, mouse
application: western blot

Western blot analysis of TNF alpha on different lysates with Rabbit anti-TNF alpha antibody (HA750897) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100ng/mL LPS for 7 hours, add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 26/50/17 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750897) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

TNF alpha
HUABIO
catalog: R1203-1

domestic rabbit polyclonal
reactivity: mouse
application: western blot, ELISA

Western Blot analysis of recombinant murine TNF-alpha using rabbit TNF alpha antibody. Western blot analysis of TNF alpha on recombinant protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1203-1, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier