ESR1
HUABIO
catalog: 0803-1
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis on MCF-7 using anti-ESR1 polyclonal antibody

Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-ESR1 antibody (0803-1) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0803-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry analysis of SK-Br-3 cells labeling ESR1 with Rabbit anti-ESR1 antibody (0803-1) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ESR1 antibody (0803-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 330 USD
to the supplier
Estrogen Receptor alpha
HUABIO
catalog: ER1803-83
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Estrogen Receptor alpha on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of Estrogen Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with Estrogen Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
quantity: 100μl
price: 330 USD
to the supplier
Phospho-Estrogen Receptor alpha (S118)
HUABIO
catalog: ET1610-32
domestic rabbit monoclonal (SC05-87)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Estrogen Receptor alpha (S118) on different lysates with Rabbit anti-Phospho-Estrogen Receptor alpha (S118) antibody (ET1610-32) at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate Lane 2: MCF7 treated with 100nM Estradiol for 30 minutes add 100ng/ml EGF for 5 minutes whole cell lysate Lane 3: MCF7 treated with 100nM Estradiol for 30 minutes then treated with 100ng/ml EGF for 5 minutes whole cell lysate Lane 4: MCF7 whole cell lysate treated with λpp for 1 hour Lane 5: MCF7 treated with 100nM Estradiol for 30 minutes add 100ng/ml EGF for 5 minutes whole cell lysate treated with λpp for 1 hour Lane 6: MCF7 treated with 100nM Estradiol for 30 minutes then treated with 100ng/ml EGF for 5 minutes whole cell lysate treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-32) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

ICC staining of Phospho-Estrogen Receptor alpha (S118) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of Phospho-Estrogen Receptor alpha (S118) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier
Estrogen Receptor alpha
HUABIO
catalog: ET7110-60
domestic rabbit monoclonal (JE26-14)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Estrogen Receptor alpha on different lysates with Rabbit anti-Estrogen Receptor alpha antibody (ET7110-60) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: T-47D cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (negative) (20 µg/Lane) Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-60) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-Estrogen Receptor alpha antibody (ET7110-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue with Rabbit anti-Estrogen Receptor alpha antibody (ET7110-60) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-60) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Estrogen Receptor alpha
HUABIO
catalog: HA721140
domestic rabbit monoclonal (PD00-04)
reactivity: human
application: immunohistochemistry - paraffin section

Application: IF-Tissue Species: Human Site: endometrium Sample: Paraffin-embedded section Antibody concentration: 1/500

Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Estrogen Receptor alpha antibody (HA721140) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721140) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Estrogen Receptor alpha antibody (HA721140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Estrogen Receptor alpha
HUABIO
catalog: IRS142RB
domestic rabbit monoclonal
reactivity: human

mIHC analysis of human breast tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Estrogen Receptor alpha antibody (IRS142RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 278.00 USD
to the supplier