domestic rabbit monoclonal (PSH08-51)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA722997) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: HT-29 cell lysate Lane 4: A549 cell lysate Lane 5: COS-1 cell lysate Lane 6: C2C12 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C6 cell lysate Lane 9: PC-12 cell lysate Lane 10: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722997) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with HA722997 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722997 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input) Lane 2: HA722997 IP in HT-29 cell lysate Lane 3: Rabbit IgG instead of HA722997 in HT-29 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 37 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-52)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA722998) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: U-2 OS cell lysate (20 µg/Lane) Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722998) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HMGCS1 antibody (HA722998) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722998) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-51)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA751226) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: HT-29 cell lysate Lane 4: A549 cell lysate Lane 5: COS-1 cell lysate Lane 6: C2C12 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C6 cell lysate Lane 9: PC-12 cell lysate Lane 10: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751226) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HMGCS1 was immunoprecipitated from 0.2 mg HT-29 cell lysate with HA751226 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751226 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input) Lane 2: HA751226 IP in HT-29 cell lysate Lane 3: Rabbit IgG instead of HA751226 in HT-29 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 37 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH08-52)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of HMGCS1 on different lysates with Rabbit anti-HMGCS1 antibody (HA751227) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: HT-29 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: U-2 OS cell lysate (20 µg/Lane) Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751227) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HMGCS1 antibody (HA751227) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751227) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier