domestic rabbit monoclonal (SY13-09)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ET1605-49) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Raji cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: MDA-MB-231 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: RAW264.7 cell lysate (20 µg/Lane) Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-49) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ET1605-49) at 1/500 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si STAT6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 1 minute 34 seconds; 4-20% SDS-PAGE gel. ET1605-49 was shown to specifically react with STAT6 in Hela-si NT cells. Weakened band was observed when Hela-si STAT6 sample was tested. Hela-si NT and Hela-si STAT6 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1605-49, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
STAT6 was immunoprecipitated from 0.5 mg Hela whole cell lysates with ET1605-49 at 2 μg/mL. Western blot was performed from the immunoprecipitate using ET1605-49 at 1/500 dilution for 45 minutes at room temperature. Goat anti-Rabbit IgG-HRP Secondary Antibody (HA1001) was used at 1:300,000 dilution for 30 minutes at room temperature. Lane 1: Hela whole cell lysates at 10 μg; Lane 2: STAT6 (ET1605-49) IP in Hela whole cell lysates; Lane 3: Rabbit IgG instead of STAT6 (ET1605-49) in Hela whole cell lysates. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (SY02-72)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ET1607-61) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 94 kDa Observed band size: 94 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-61) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ET1607-61) at 1/500 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si STAT6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 94 kDa Observed band size: 94 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. ET1607-61 was shown to specifically react with STAT6 in Hela-si NT cells. Weakened band was observed when Hela-si STAT6 sample was tested. Hela-si NT and Hela-si STAT6 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1607-61, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-STAT6 antibody (ET1607-61) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH06-98)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA722776) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA722776) at 1/2,000 dilution. Lane 1: J774A.1 cell lysate Lane 2: J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722776) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH06-98)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA751125) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751125) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA751125) at 1/2,000 dilution. Lane 1: J774A.1 cell lysate Lane 2: J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751125) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier