Alpha 1 antitrypsin
HUABIO
catalog: HA723819

domestic rabbit monoclonal (PSH16-21)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Alpha 1 antitrypsin on different lysates with Rabbit anti-Alpha 1 antitrypsin antibody (HA723819) at 1/50,000 dilution. Lane 1: Mouse liver tissue lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (20 µg/Lane) Lane 4: Rat spleen ver tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723819) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alpha 1 antitrypsin antibody (HA723819) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723819) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Mouse Alpha 1 antitrypsin
HUABIO
catalog: HA725146

domestic rabbit monoclonal (PSH16-20)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse Alpha 1 antitrypsin matched pair antibodies Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725146) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Alpha 1 antitrypsin protein (HA211161) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA725147, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Alpha 1 antitrypsin in mouse serum. Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] The concentrations of Alpha 1 antitrypsin were measured in duplicates, interpolated from the Alpha 1 antitrypsin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha 1 antitrypsin concentration was determined to be 280 ug/ml in mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse Alpha 1 antitrypsin
HUABIO
catalog: HA725147

domestic rabbit monoclonal (PSH16-21)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse Alpha 1 antitrypsin matched pair antibodies Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725146) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Alpha 1 antitrypsin protein (HA211161) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA725147, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Alpha 1 antitrypsin in mouse serum. Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] The concentrations of Alpha 1 antitrypsin were measured in duplicates, interpolated from the Alpha 1 antitrypsin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha 1 antitrypsin concentration was determined to be 280 ug/ml in mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse Alpha 1 antitrypsin
HUABIO
catalog: HA725148B

domestic rabbit monoclonal (PSH16-21)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse Alpha 1 antitrypsin matched pair antibodies Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725146) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Alpha 1 antitrypsin protein (HA211161) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA725147, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Alpha 1 antitrypsin in mouse serum. Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] The concentrations of Alpha 1 antitrypsin were measured in duplicates, interpolated from the Alpha 1 antitrypsin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Alpha 1 antitrypsin concentration was determined to be 280 ug/ml in mouse serum.
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse Alpha 1 antitrypsin
HUABIO
catalog: HA725148H

domestic rabbit monoclonal (PSH16-21)
reactivity: mouse
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Mouse Alpha 1 antitrypsin matched pair antibodies Capture: HA725146, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-20] Detector: HA725147, Mouse Alpha 1 antitrypsin Rabbit mAb [PSH16-21] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725146) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Alpha 1 antitrypsin protein (HA211161) starting from 1,000,000 pg/ml to 0 pg/ml and detect antibody (HA725147, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Alpha 1 antitrypsin
HUABIO
catalog: HA751592

domestic rabbit monoclonal (PSH16-21)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Alpha 1 antitrypsin on different lysates with Rabbit anti-Alpha 1 antitrypsin antibody (HA751592) at 1/50,000 dilution. Lane 1: Mouse liver tissue lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (20 µg/Lane) Lane 4: Rat spleen ver tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751592) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alpha 1 antitrypsin antibody (HA751592) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751592) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier