HUABIO Sox2 antibody

SOX2
HUABIO
catalog: HA720179F

domestic rabbit monoclonal (PO00-28)
reactivity: human, mouse
application: flow cytometry

Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (HA720179F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (HA720179F) at 1/50 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Flow cytometric analysis of F9 cells labeling SOX2. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with SOX2 (HA720179F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 429.00 USD
to the supplier

SOX2
HUABIO
catalog: HA720180F

domestic rabbit monoclonal (PO00-28)
reactivity: human, mouse
application: flow cytometry

Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (HA720180F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (HA720180F) at 1/50 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Flow cytometric analysis of F9 cells labeling SOX2. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with SOX2 (HA720180F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 429.00 USD
to the supplier

SOX2
HUABIO
catalog: HA721155

domestic rabbit monoclonal (PO00-28)
reactivity: human, mouse, rat
application: western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: E14.5 embryonic brain Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: E14.5 embryo Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: E14.5 embryonic cartilage Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

SOX2
HUABIO
catalog: IRS240RB

domestic rabbit monoclonal
reactivity: mouse

mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-SOX2 antibody (IRS240RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 278.00 USD
to the supplier

SOX2
HUABIO
catalog: R1106-1

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (R1106-1) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: F9 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Human brain tissue lysate (40 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1106-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

SOX2
HUABIO
catalog: HA601396

domestic rabbit polyclonal (PO00-28)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: E14.5 embryonic brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo lung tissue with Rat anti-SOX2 antibody (HA601396) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601396) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier

SOX2
HUABIO
catalog: HA601499

domestic rabbit polyclonal (PO00-28)
reactivity: human, mouse
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of SOX2 on different lysates with Chicken anti-SOX2 antibody (HA601499) at 1/5,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 34 kDa Observed band size: 36 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601499) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Chicken IgY - HRP Secondary Antibody (HA1046) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

SOX2
HUABIO
catalog: HA601500

domestic rabbit polyclonal (PO00-28)
reactivity: human, mouse
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of SOX2 on different lysates with Guinea pig anti-SOX2 antibody (HA601500) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601500) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

SOX2
HUABIO
catalog: HA610236

domestic rabbit polyclonal (PO00-28)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section