domestic rabbit monoclonal (PSH16-72)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723874) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723875, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native L-selectin in mouse serum . Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Interpolated concentration of native L-selectin was measured in duplicate at different sample concentrations and interpolated from the L-selectin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean L-selectin concentration was determined to be 803,180 pg/ml in mouse serum.
Interpolated concentrations of spiked L-selectin in cell culture media samples. Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] The concentrations of L-selectin were measured in duplicates, interpolated from the L-selectin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-73)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723874) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723875, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies Capture: HA723876, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-74] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723876) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723875, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native L-selectin in mouse serum . Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Interpolated concentration of native L-selectin was measured in duplicate at different sample concentrations and interpolated from the L-selectin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean L-selectin concentration was determined to be 803,180 pg/ml in mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-73)
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723874) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723875, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies Capture: HA723876, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-74] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723876) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723875, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native L-selectin in mouse serum . Capture: HA723874, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-72] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Interpolated concentration of native L-selectin was measured in duplicate at different sample concentrations and interpolated from the L-selectin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean L-selectin concentration was determined to be 803,180 pg/ml in mouse serum.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-74)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of Mouse L-Selectin / CD62L matched pair antibodies Capture: HA723876, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-74] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723876) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse L-Selectin / CD62L protein (HA211126) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723875, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native L-selectin in mouse serum . Capture: HA723876, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-74] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] Interpolated concentration of native L-selectin was measured in duplicate at different sample concentrations and interpolated from the L-selectin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean L-selectin concentration was determined to be 771,994 pg/ml in mouse serum.
Interpolated concentrations of spiked L-selectin in cell culture media samples. Capture: HA723876, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-74] Detector: HA723875, Mouse L-Selectin / CD62L Rabbit mAb [PSH16-73] The concentrations of L-selectin were measured in duplicates, interpolated from the L-selectin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier