domestic rabbit monoclonal (JM03-45)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of RANTES on mouse thymus lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
ICC staining of RANTES in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-70, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of RANTES in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-70, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier
rat monoclonal
reactivity: human, mouse
application: ELISA
reactivity: human, mouse
application: ELISA
domestic rabbit monoclonal (PSH14-15)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of mouse CCL5 matched pair antibodies Capture: HA725046, Mouse CCL5/RANTES Rabbit mAb [PSH14-15] Detector: HA725047, Mouse CCL5/RANTES Rabbit mAb [PSH14-16] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725046) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CCL5/RANTES protein (HA211095) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725047, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL5 in mouse samples. Capture: HA725046, Mouse CCL5/RANTES Rabbit mAb [PSH14-15] Detector: HA725047, Mouse CCL5/RANTES Rabbit mAb [PSH14-16] Interpolated concentration of native CCL5 was measured in duplicate at different sample concentrations and interpolated from the CCL5 standard curves. Undiluted samples were 100% mouse serum. The mean CCL5 concentration was determined to be 162 pg/mL in mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-16)
reactivity: mouse
application: ELISA
reactivity: mouse
application: ELISA
Sandwich ELISA analysis of mouse CCL5 matched pair antibodies Capture: HA725046, Mouse CCL5/RANTES Rabbit mAb [PSH14-15] Detector: HA725047, Mouse CCL5/RANTES Rabbit mAb [PSH14-16] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725046) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CCL5/RANTES protein (HA211095) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725047, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL5 in mouse samples. Capture: HA725046, Mouse CCL5/RANTES Rabbit mAb [PSH14-15] Detector: HA725047, Mouse CCL5/RANTES Rabbit mAb [PSH14-16] Interpolated concentration of native CCL5 was measured in duplicate at different sample concentrations and interpolated from the CCL5 standard curves. Undiluted samples were 100% mouse serum. The mean CCL5 concentration was determined to be 162 pg/mL in mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH14-16)
reactivity: mouse
conjugate: biotin
application: ELISA
reactivity: mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of mouse CCL5 matched pair antibodies Capture: HA725046, Mouse CCL5/RANTES Rabbit mAb [PSH14-15] Detector: HA725047, Mouse CCL5/RANTES Rabbit mAb [PSH14-16] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725046) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CCL5/RANTES protein (HA211095) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725047, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL5 in mouse samples. Capture: HA725046, Mouse CCL5/RANTES Rabbit mAb [PSH14-15] Detector: HA725047, Mouse CCL5/RANTES Rabbit mAb [PSH14-16] Interpolated concentration of native CCL5 was measured in duplicate at different sample concentrations and interpolated from the CCL5 standard curves. Undiluted samples were 100% mouse serum. The mean CCL5 concentration was determined to be 162 pg/mL in mouse serum.
quantity: 100μl
price: 409.00 USD
to the supplier