domestic rabbit monoclonal (PSH12-45)
reactivity: mouse
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse CCL2 matched pair antibodies
Capture: HA725040, Mouse CCL2 Rabbit mAb [PSH12-44]
Detector: HA725041, Mouse CCL2 Rabbit mAb [PSH12-45]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725040) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CCL2 protein (HA210775) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725041, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL2 in J774A.1 cell culture supernatant.
Capture: HA725040, Mouse CCL2 Rabbit mAb [PSH12-44]
Detector: HA725041, Mouse CCL2 Rabbit mAb [PSH12-45]
J774A.1 were stimulated with 20 ng/ml IFN-γ and 1 μg/ml LPS incubated for 1 day. The concentrations of CCL2 were measured in duplicate and interpolated from the CCL2 standard curves. Undiluted samples are IFN-γ and LPS stimulated J774A.1 cell culture supernatant 1.56% and unstimulated J774A.1 cell culture supernatant 50%. The mean CCL2 concentration was determined to be 13,358 pg/ml in IFN-γ and LPS stimulated J774A.1 cell culture supernatant and 1,679 pg/ml in the unstimulated J774A.1 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
