S100 beta
HUABIO
catalog: ET1610-3

domestic rabbit monoclonal (SC57-02)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1:500

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA601098

mouse monoclonal (PD00-11)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of S100 beta on different lysates with Mouse anti-S100 beta antibody (HA601098) at 1/2,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: A-172 cell lysate (negative) Lane 3: Mouse brain tissue lysate Lane 4: Mouse brain tissue lysate (no heat) Lane 5: Rat brain tissue lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601098) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue with Mouse anti-S100 beta antibody (HA601098) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601098) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human meningioma tissue with Mouse anti-S100 beta antibody (HA601098) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601098) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA720125F

domestic rabbit monoclonal (SC57-02)
reactivity: human, mouse, rat

Immunofluorescence analysis of paraffin-embedded human malignant melanoma tissue labeling S100 beta. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720125F S100 beta (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded human meningioma tissue labeling S100 beta. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720125F S100 beta (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain.
quantity: 100μl
price: 429.00 USD
to the supplier

S100B
HUABIO
catalog: HA723728

domestic rabbit monoclonal (SC57-02)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of Human S100B matched pair antibodies Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723728) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted S100B protein starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723729, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native S100B in SK-MEL-28 and T-47D extract samples based on a 1,000 µg/ml extract load. Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] The concentrations of S100B were measured in triplicates, interpolated from the S100B standard curve and corrected for sample dilution. Undiluted samples are SK-MEL-28 extract 0.25% and T-47D extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean S100B concentration was determined to be 1,536 ng/ml in SK-MEL-28 extract and undetectable in T-47D extract.

Interpolated concentrations of spiked S100B in serum-free media samples. Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] The concentrations of S100B were measured in duplicates, interpolated from the S100B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum-free media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

S100B
HUABIO
catalog: HA723729

domestic rabbit monoclonal (PSH15-25)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of Human S100B matched pair antibodies Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723728) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted S100B protein starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723729, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native S100B in SK-MEL-28 and T-47D extract samples based on a 1,000 µg/ml extract load. Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] The concentrations of S100B were measured in triplicates, interpolated from the S100B standard curve and corrected for sample dilution. Undiluted samples are SK-MEL-28 extract 0.25% and T-47D extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean S100B concentration was determined to be 1,536 ng/ml in SK-MEL-28 extract and undetectable in T-47D extract.

Interpolated concentrations of spiked S100B in serum-free media samples. Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] The concentrations of S100B were measured in duplicates, interpolated from the S100B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum-free media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

S100B
HUABIO
catalog: HA723730B

domestic rabbit monoclonal (PSH15-25)
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human S100B matched pair antibodies Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723728) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted S100B protein starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723729, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native S100B in SK-MEL-28 and T-47D extract samples based on a 1,000 µg/ml extract load. Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] The concentrations of S100B were measured in triplicates, interpolated from the S100B standard curve and corrected for sample dilution. Undiluted samples are SK-MEL-28 extract 0.25% and T-47D extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean S100B concentration was determined to be 1,536 ng/ml in SK-MEL-28 extract and undetectable in T-47D extract.

Interpolated concentrations of spiked S100B in serum-free media samples. Capture: HA723728, Human S100B Rabbit mAb [SC57-02] Detector: HA723729, Human S100B Rabbit mAb [PSH15-25] The concentrations of S100B were measured in duplicates, interpolated from the S100B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum-free media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA723900

domestic rabbit monoclonal (PSH17-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-S100 beta antibody (HA723900) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723900) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue with Rabbit anti-S100 beta antibody (HA723900) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723900) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA750209

domestic rabbit monoclonal (SC57-02)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required

Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1:500

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-S100 beta antibody (HA750209) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750209) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA751617

domestic rabbit monoclonal (PSH17-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-S100 beta antibody (HA751617) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751617) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue with Rabbit anti-S100 beta antibody (HA751617) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751617) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA601550

domestic rabbit polyclonal (SC57-02)
reactivity: mouse
application: immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA601551

domestic rabbit polyclonal (SC57-02)
reactivity: mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-S100 beta antibody (HA601551) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601551) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA601552

domestic rabbit polyclonal (SC57-02)
reactivity: mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 (S100 beta, HA601552, Rat, red); 1/200 (SOX2, HA601499, Chicken, green) Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rat anti-S100 beta antibody (HA601552) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601552) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA610365

domestic rabbit polyclonal (SC57-02)
reactivity: mouse
application: immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA610366

domestic rabbit polyclonal (SC57-02)
reactivity: mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-S100 beta antibody (HA610366) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610366) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

S100 beta
HUABIO
catalog: HA610367

domestic rabbit polyclonal (SC57-02)
reactivity: mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 (S100 beta, HA610367, Rat, red); 1/200 (SOX2, HA601499, Chicken, green) Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rat anti-S100 beta antibody (HA610367) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610367) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier