HUABIO Rela antibody

NF-kB p65
HUABIO
catalog: ER0815

domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NF-κB p65 on different lysates using anti-NF-κB p65 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: PC12 cell lysate Lane 4: Mouse embryonic stem cell lysate Lane 5: NIH/3T3 cell lysate

Western blot analysis of NF-kB p65 on zebrafish lysates with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/2,000 dilution. Lysates at 20 µg/Lane. Predicted band size: 60kDa Observed band size: 70 kDa Exposure time: 3min20s; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0815) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier

NF-kB p65
HUABIO
catalog: ET1603-12

domestic rabbit monoclonal (SZ10-04)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (ET1603-12) at 1/5,000 dilution. Lane 1: Wild-type HeLa cell lysate Lane 2: HeLa KO cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-12) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (ET1603-12) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 49 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-12) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (ET1603-12) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NF-kB p65 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-12) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

NF-kB p65
HUABIO
catalog: HA500402

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA500402) at 1/50,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: PC-12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500402) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SH-SY5Y cells labeling NF-kB p65 with Rabbit anti-NF-kB p65 antibody (HA500402) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-kB p65 antibody (HA500402) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of PC-12 cells labeling NF-kB p65 with Rabbit anti-NF-kB p65 antibody (HA500402) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-kB p65 antibody (HA500402) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier

NF-kB p65
HUABIO
catalog: HA721307

domestic rabbit monoclonal (PSH0-27)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA721307) at 1/500 dilution. Lane 1: HeLa cell lysate (control) (35 µg/Lane) Lane 2: HeLa-RELA-KO (1) (35 µg/Lane) Lane 3: HeLa-RELA-KO (2) (35 µg/Lane) Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721307) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-NF-kB p65 antibody (HA721307) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721307) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-NF-kB p65 (S536)
HUABIO
catalog: HA723223

domestic rabbit monoclonal (PSH10-58)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-NF-kB p65 (S536) on different lysates with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA723223) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 10 minutes cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 treated with 100nM Calyculin A and 20ng/mL TNF-α for 10 minutes cell lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723223) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells untreated / treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes labeling Phospho-NF-kB p65 (S536) with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA723223) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA723223) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis of Phospho-NF-kB p65 (S536) on different lysates with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA723223) at 1/5,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723223) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

NF-kB p65
HUABIO
catalog: HA750058

domestic rabbit monoclonal (SZ10-04)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750058) at 1/5,000 dilution. Lane 1: Wild-type HeLa cell lysate Lane 2: HeLa KO cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750058) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750058) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 49 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750058) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750058) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NF-kB p65 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750058) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

NF-kB p65
HUABIO
catalog: HA750601

domestic rabbit monoclonal (PSH0-27)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/500 dilution. Lane 1: HeLa cell lysate (control) (35 µg/Lane) Lane 2: HeLa-RELA-KO (1) (35 µg/Lane) Lane 3: HeLa-RELA-KO (2) (35 µg/Lane) Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750601) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-NF-kB p65 (S536)
HUABIO
catalog: HA751361

domestic rabbit monoclonal (PSH10-58)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-NF-kB p65 (S536) on different lysates with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA751361) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 10 minutes cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 treated with 100nM Calyculin A and 20ng/mL TNF-α for 10 minutes cell lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751361) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells untreated / treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes labeling Phospho-NF-kB p65 (S536) with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA751361) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA751361) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis of Phospho-NF-kB p65 (S536) on different lysates with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA751361) at 1/5,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751361) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Anti-NF-κB p65 Antibody [SZ10-04]
HUABIO
catalog: ET1603-12TR

domestic rabbit monoclonal (SZ10-04)
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

quantity: 20 uL
price: 99 USD
to the supplier