domestic rabbit monoclonal (PSH09-21)
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry
Western blot analysis of Eph receptor A2 on different lysates with Rabbit anti-Eph receptor A2 antibody (HA723079) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 108 kDa Observed band size: 130 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723079) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A549 cells labeling Eph receptor A2 with Rabbit anti-Eph receptor A2 antibody (HA723079) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor A2 antibody (HA723079) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Eph receptor A2 was immunoprecipitated from 0.2 mg A549 cell lysate with HA723079 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723079 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: HA723079 IP in A549 cell lysate Lane 3: Rabbit IgG instead of HA723079 in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-12)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human EphA2 matched pair antibodies Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723714) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human EphA2 protein (HA211075) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723715, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native EphA2 in HEK293 and SK-MEL-28 extract samples based on a 1,000 µg/ml extract load. Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curve and corrected for sample dilution. Undiluted samples are HEK293 extract 40% and SK-MEL-28 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EphA2 concentration was determined to be 15,046 pg/ml in HEK293 extract and undetectable in SK-MEL-28 extract.
Interpolated concentrations of spiked EphA2 in human cell culture media samples. Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-13)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human EphA2 matched pair antibodies Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723714) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human EphA2 protein (HA211075) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723715, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native EphA2 in HEK293 and SK-MEL-28 extract samples based on a 1,000 µg/ml extract load. Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curve and corrected for sample dilution. Undiluted samples are HEK293 extract 40% and SK-MEL-28 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EphA2 concentration was determined to be 15,046 pg/ml in HEK293 extract and undetectable in SK-MEL-28 extract.
Interpolated concentrations of spiked EphA2 in human cell culture media samples. Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-13)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human EphA2 matched pair antibodies Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723714) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human EphA2 protein (HA211075) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723715, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native EphA2 in HEK293 and SK-MEL-28 extract samples based on a 1,000 µg/ml extract load. Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curve and corrected for sample dilution. Undiluted samples are HEK293 extract 40% and SK-MEL-28 extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EphA2 concentration was determined to be 15,046 pg/ml in HEK293 extract and undetectable in SK-MEL-28 extract.
Interpolated concentrations of spiked EphA2 in human cell culture media samples. Capture: HA723714, Human EphA2 Rabbit mAb [PSH15-12] Detector: HA723715, Human EphA2 Rabbit mAb [PSH15-13] The concentrations of EphA2 were measured in duplicates, interpolated from the EphA2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH09-21)
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry
Western blot analysis of Eph receptor A2 on different lysates with Rabbit anti-Eph receptor A2 antibody (HA751275) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 108 kDa Observed band size: 130 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751275) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A549 cells labeling Eph receptor A2 with Rabbit anti-Eph receptor A2 antibody (HA751275) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor A2 antibody (HA751275) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Eph receptor A2 was immunoprecipitated from 0.2 mg A549 cell lysate with HA751275 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751275 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: HA751275 IP in A549 cell lysate Lane 3: Rabbit IgG instead of HA751275 in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier