Presenilin 1 / PS-1
HUABIO
catalog: ER1915-40

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (ER1915-40) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Neuro-2a cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 53 kDa Observed band size: 28 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1915-40) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier

Presenilin 1 / PS-1
HUABIO
catalog: HA500219

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Presenilin 1 / PS-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500219, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Raji cell lysate Lane 3: MCF-7 cell lysate Lane 4: Daudi cell lysate

Western blot analysis of Presenilin 1 / PS-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500219, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate

ICC staining of Presenilin 1 / PS-1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500219, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier

Presenilin 1 / PS-1
HUABIO
catalog: HA722995

domestic rabbit monoclonal (PSH08-49)
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Presenilin 1 / PS-1 antibody (HA722995) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722995) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Presenilin 1 / PS-1
HUABIO
catalog: HA72$3301

domestic rabbit monoclonal (PSH11-19)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

quantity: 100μl
price: 385.00 USD
to the supplier

Presenilin 1 / PS-1
HUABIO
catalog: HA751224

domestic rabbit monoclonal (PSH08-49)
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Presenilin 1 / PS-1 antibody (HA751224) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751224) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Presenilin 1 / PS-1
HUABIO
catalog: HA751400

domestic rabbit monoclonal (PSH11-19)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (HA751400) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: MEF cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: HL-60 cell lysate (20 µg/Lane) Lane 6: Daudi cell lysate (20 µg/Lane) Lane 7: Neuro-2a cell lysate (20 µg/Lane) Lane 8: Mouse brain tissue lysate (40 µg/Lane) Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 10: Rat brain tissue lysate (40 µg/Lane) Lane 11: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 53 kDa Observed band size: 18 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751400) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA751400 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751400 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA751400 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA751400 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier