HUABIO Ngfr antibody

CD271
HUABIO
catalog: 1003-9

domestic rabbit polyclonal
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD271 on different lysates with Rabbit anti-CD271 antibody (1003-9) at 1/1,000 dilution. Lane 1: F9 cell lysate Lane 2: PC-12 cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1003-9) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of L6 cells labeling CD271 with Rabbit anti-CD271 antibody (1003-9) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD271 antibody (1003-9) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 330 USD
to the supplier

p75 NGF Receptor
HUABIO
catalog: ET1601-22-50UL

domestic rabbit monoclonal (SA39-02)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of p75 NGF Receptor on Neuro-2a cell lysates with Rabbit anti-p75 NGF Receptor antibody (ET1601-22) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of p75 NGF Receptor on PC-12 cell lysates with Rabbit anti-p75 NGF Receptor antibody (ET1601-22) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-22) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of p75 NGF Receptor on different lysates with Rabbit anti-p75 NGF Receptor antibody (ET1601-22) at 1/2,000 dilution. Lane 1: PC-12-si NT cell lysate Lane 2: PC-12-si p75 NGF Receptor cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier

p75 NGF Receptor
HUABIO
catalog: HA601436

rat monoclonal (PSH13-26)
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain (medial septum) Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: colon Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of p75 NGF Receptor on different lysates with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601436) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

p75 NGF Receptor
HUABIO
catalog: HA610274

rat monoclonal (PSH13-26)
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain (medial septum) Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: colon Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of p75 NGF Receptor on different lysates with Rat anti-p75 NGF Receptor antibody (HA610274) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610274) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier

p75 NGF Receptor
HUABIO
catalog: HA720190F

domestic rabbit monoclonal (SA39-02)
reactivity: human, mouse, rat

Immunocytochemistry analysis of PC-12 cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA720190F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA720190F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 429.00 USD
to the supplier

p75 NGF Receptor
HUABIO
catalog: HA750014

Western blot analysis of p75 NGF Receptor on Neuro-2a cell lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of p75 NGF Receptor on PC-12 cell lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of p75 NGF Receptor on different lysates with Rabbit anti-p75 NGF Receptor antibody (HA750014) at 1/2,000 dilution. Lane 1: PC-12-si NT cell lysate Lane 2: PC-12-si p75 NGF Receptor cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 70 kDa Exposure time: 2 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750014) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

p75 NGF Receptor
HUABIO
catalog: HA723532

domestic rabbit polyclonal (PSH13-26)
reactivity: mouse
application: immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier

p75 NGF Receptor
HUABIO
catalog: HA751462

domestic rabbit polyclonal (PSH13-26)
reactivity: mouse
application: immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier