NF-L
HUABIO
catalog: ER65439
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (ER65439) at 1/100,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat hippocampus tissue lysate Lane 4: Human brain tissue lysate Lysates/proteins at 5 µg/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65439) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (ER65439) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (ER65439) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-NF-L antibody (ER65439) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65439) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
NF-L
HUABIO
catalog: HA721498
domestic rabbit monoclonal (PSH0-77)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (HA721498) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Mouse liver tissue lysate (negative) Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721498) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (HA721498) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721498) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NF-L antibody (HA721498) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721498) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
NF-L
HUABIO
catalog: HA721538
domestic rabbit monoclonal (PS02-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (HA721538) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Mouse liver tissue lysate (negative) Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721538) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
Human/Mouse/Rat NF-L
HUABIO
catalog: HA723$330
domestic rabbit monoclonal (PSH11-41)
reactivity: human, mouse, rat
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
Human/Mouse/Rat NF-L
HUABIO
catalog: HA723331
domestic rabbit monoclonal (PSH11-42)
reactivity: human, mouse, rat
application: ELISA

Standard curve of human NF-L matched pair antibodies Sandwich ELISA analysis of human NF-L matched pair antibodiesElisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723$330) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NF-L protein (HA211087) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723331, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NF-L in mouse brain, rat brain and U-87 MG extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NF-L was measured in duplicate at different sample concentrations and interpolated from the NF-L standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NF-L concentration was determined to be 643,742 pg/mL in mouse brain and 127,718 pg/mL in rat brain tissue extract. There was no detectable signal in U-87 MG cell extract.

Interpolated concentrations of spiked NF-L in cell culture media samples. The concentrations of NF-L were measured in duplicates, interpolated from the NF-L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human/Mouse/Rat NF-L
HUABIO
catalog: HA723332B
domestic rabbit monoclonal (PSH11-42)
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA

Standard curve of human NF-L matched pair antibodies Sandwich ELISA analysis of human NF-L matched pair antibodiesElisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723$330) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NF-L protein (HA211087) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723332B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NF-L in mouse brain, rat brain and U-87 MG extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NF-L was measured in duplicate at different sample concentrations and interpolated from the NF-L standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NF-L concentration was determined to be 643,742 pg/mL in mouse brain and 127,718 pg/mL in rat brain tissue extract. There was no detectable signal in U-87 MG cell extract.

Interpolated concentrations of spiked NF-L in cell culture media samples. The concentrations of NF-L were measured in duplicates, interpolated from the NF-L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
Human/Mouse/Rat NF-L
HUABIO
catalog: HA723333
domestic rabbit monoclonal (PSH11-43)
reactivity: human, mouse, rat
application: ELISA

Standard curve of human NF-L matched pair antibodies Sandwich ELISA analysis of human NF-L matched pair antibodiesElisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723333) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NF-L protein (HA211087) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723334, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NF-L in mouse brain, rat brain and U-87 MG extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NF-L was measured in duplicate at different sample concentrations and interpolated from the NF-L standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NF-L concentration was determined to be 934,886 pg/mL in mouse brain and 406.883 pg/mL in rat brain tissue extract. There was no detectable signal in U-87 MG cell extract.

Interpolated concentrations of spiked NF-L in cell culture media samples. The concentrations of NF-L were measured in duplicates, interpolated from the NF-L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human/Mouse/Rat NF-L
HUABIO
catalog: HA723334
domestic rabbit monoclonal (PSH11-44)
reactivity: human, mouse, rat
application: ELISA

Standard curve of human NF-L matched pair antibodies Sandwich ELISA analysis of human NF-L matched pair antibodiesElisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723333) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NF-L protein (HA211087) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723334, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NF-L in mouse brain, rat brain and U-87 MG extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NF-L was measured in duplicate at different sample concentrations and interpolated from the NF-L standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NF-L concentration was determined to be 934,886 pg/mL in mouse brain and 406.883 pg/mL in rat brain tissue extract. There was no detectable signal in U-87 MG cell extract.

Interpolated concentrations of spiked NF-L in cell culture media samples. The concentrations of NF-L were measured in duplicates, interpolated from the NF-L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human/Mouse/Rat NF-L
HUABIO
catalog: HA723335B
domestic rabbit monoclonal (PSH11-44)
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA

Standard curve of human NF-L matched pair antibodies Sandwich ELISA analysis of human NF-L matched pair antibodiesElisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723333) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human NF-L protein (HA211087) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723335B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NF-L in mouse brain, rat brain and U-87 MG extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NF-L was measured in duplicate at different sample concentrations and interpolated from the NF-L standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NF-L concentration was determined to be 934,886 pg/mL in mouse brain and 406.883 pg/mL in rat brain tissue extract. There was no detectable signal in U-87 MG cell extract.

Interpolated concentrations of spiked NF-L in cell culture media samples. The concentrations of NF-L were measured in duplicates, interpolated from the NF-L standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
NF-L
HUABIO
catalog: HA750657
domestic rabbit monoclonal (PSH0-77)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (HA750657) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Mouse liver tissue lysate (negative) Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750657) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (HA750657) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750657) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NF-L antibody (HA750657) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750657) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
NF-L
HUABIO
catalog: HA750678
domestic rabbit monoclonal (PS02-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (HA750678) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Mouse liver tissue lysate (negative) Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750678) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier
NF-L
HUABIO
catalog: HA601409
domestic rabbit polyclonal (PS02-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-NF-L antibody (HA601409) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601409) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-L antibody (HA601409) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601409) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
NF-L
HUABIO
catalog: HA601448
domestic rabbit polyclonal (PS02-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Mouse anti-NF-L antibody (HA601448) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601448) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
NF-L
HUABIO
catalog: HA601463
domestic rabbit polyclonal (PS02-10)
reactivity: mouse, rat
application: immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μl
price: 360.00 USD
to the supplier
NF-L
HUABIO
catalog: HA601472
domestic rabbit polyclonal (PS02-10)
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of NF-L on different lysates with Chicken anti-NF-L antibody (HA601472) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601472) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
NF-L
HUABIO
catalog: HA610249
domestic rabbit polyclonal (PS02-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-NF-L antibody (HA610249) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610249) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-L antibody (HA610249) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610249) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier
NF-L
HUABIO
catalog: HA610282
domestic rabbit polyclonal (PS02-10)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Mouse anti-NF-L antibody (HA610282) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610282) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier
NF-L
HUABIO
catalog: HA610297
domestic rabbit polyclonal (PS02-10)
reactivity: mouse, rat
application: immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
quantity: 100μg
price: 649.00 USD
to the supplier
NF-L
HUABIO
catalog: HA610306
domestic rabbit polyclonal (PS02-10)
reactivity: mouse, rat
application: western blot, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

Western blot analysis of NF-L on different lysates with Chicken anti-NF-L antibody (HA610306) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610306) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier