domestic rabbit monoclonal (SR38-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (PSD95, ET1602-20, red); 1:500 (Iba1, HA601376, green) Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1: 1,000 (PSD95, ET1602-20, red); 1:500 (VGLUT2, HA601393, green) Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 1,000 (PSD95, ET1602-20, red); 1:500 (VGLUT2, HA601393, green) Antigen retrieval: Not required
quantity: 50μl
price: 205.00 USD
to the supplier
domestic rabbit monoclonal (SR38-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (PSD95, HA750035, red); 1:500 (Iba1, HA601376, green) Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1: 1,000 (PSD95, HA750035, red); 1:500 (VGLUT2, HA601393, green) Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 1,000 (PSD95, HA750035, red); 1:500 (VGLUT2, HA601393, green) Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier
mouse monoclonal (B5-E6)
reactivity: human, mouse, rat
application: western blot, flow cytometry
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of PSD95 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-4, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHG-44 cell lysate Lane 2: A172 cell lysate Lane 3: N2A cell lysate
Immunocytochemistry analysis of Neuro-2a cells labeling PSD95 with Mouse anti-PSD95 antibody (M1511-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PSD95 antibody (M1511-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of PSD95 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-4, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis on different cell lysates using anti- PSD95 rabbit polyclonal antibodies.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- PSD95 rabbit polyclonal antibody.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- PSD95 rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier