MMP-9
HUABIO
catalog: ET1704-69-50UL
domestic rabbit monoclonal (JA80-73)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (ET1704-69) at 1/5,000 dilution. Lane 1: THP-1 whole cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 200nM TPA for 10 minutes whole cell lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 78 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MMP9 on different lysates with Rabbit anti-MMP9 antibody (ET1704-69) at 1/5,000 dilution. Lane 1: A549-si NT+TPA(80nM 24h) cell lysate, 15 µg/Lane Lane 2: A549-si MMP9#1+TPA(80nM 24h) cell lysate, 15 µg/Lane Lane 3: A549-si MMP9#2+TPA(80nM 24h) cell lysate, 15 µg/Lane Predicted band size: 78 kDa Observed band size: 78-92 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-69, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MMP-9 antibody (ET1704-69) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-69) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier
Mouse MMP-9
HUABIO
catalog: HA723784
domestic rabbit monoclonal (PSH15-79)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse MMP-9 matched pair antibodies Capture: HA723784, Mouse MMP-9 Rabbit mAb [PSH15-79] Detector: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723784) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-9 protein (HA211045) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723785, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MMP-9 in mouse serum samples. Capture: HA723784, Mouse MMP-9 Rabbit mAb [PSH15-79] Detector: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] The concentrations of MMP-9 were measured in triplicates, interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean MMP-9 concentration was determined to be 249.5 ng/ml in mouse serum.

Interpolated concentrations of native MMP-9 in mouse LPS unstimulated and stimulated J774A.1 cell culture supernatant. Capture: HA723784, Mouse MMP-9 Rabbit mAb [PSH15-79] Detector: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] J774A.1 cells were stimulated with 2.5 µg/ml LPS or vehicle control and incubated for 1 day. The concentrations of MMP-9 measured in duplicate and interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50% and stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-9 concentration was determined to be 15,121 pg/ml in LPS stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse MMP-9
HUABIO
catalog: HA723785
domestic rabbit monoclonal (PSH15-80)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse MMP-9 matched pair antibodies Capture: HA723784, Mouse MMP-9 Rabbit mAb [PSH15-79] Detector: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723784) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-9 protein (HA211045) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723785, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Mouse MMP-9 matched pair antibodies Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723785) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-9 protein (HA211045) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723787, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MMP-9 in mouse serum samples. Capture: HA723784, Mouse MMP-9 Rabbit mAb [PSH15-79] Detector: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] The concentrations of MMP-9 were measured in triplicates, interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean MMP-9 concentration was determined to be 249.5 ng/ml in mouse serum.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse MMP-9
HUABIO
catalog: HA723786B
domestic rabbit monoclonal (PSH15-80)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse MMP-9 matched pair antibodies Capture: HA723784, Mouse MMP-9 Rabbit mAb [PSH15-79] Detector: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723784) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-9 protein (HA211045) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723785, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MMP-9 in mouse serum samples. Capture: HA723784, Mouse MMP-9 Rabbit mAb [PSH15-79] Detector: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] The concentrations of MMP-9 were measured in triplicates, interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean MMP-9 concentration was determined to be 249.5 ng/ml in mouse serum.

Interpolated concentrations of native MMP-9 in mouse LPS unstimulated and stimulated J774A.1 cell culture supernatant. Capture: HA723784, Mouse MMP-9 Rabbit mAb [PSH15-79] Detector: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] J774A.1 cells were stimulated with 2.5 µg/ml LPS or vehicle control and incubated for 1 day. The concentrations of MMP-9 measured in duplicate and interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50% and stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-9 concentration was determined to be 15,121 pg/ml in LPS stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.
quantity: 100μl
price: 409.00 USD
to the supplier
Mouse MMP-9
HUABIO
catalog: HA723787
domestic rabbit monoclonal (PSH15-81)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse MMP-9 matched pair antibodies Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723785) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-9 protein (HA211045) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723787, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MMP-9 in mouse serum samples. Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] The concentrations of MMP-9 were measured in duplicates, interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-9 concentration was determined to be 370.1 ng/ml in mouse serum.

Interpolated concentrations of native MMP-9 in mouse LPS unstimulated and stimulated J774A.1 cell culture supernatant. Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] J774A.1 cells were stimulated with 2.5 µg/ml LPS or vehicle control and incubated for 1 day. The concentrations of MMP-9 measured in duplicate and interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50% and stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-9 concentration was determined to be 21,179 pg/ml in LPS stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.
quantity: 100μl
price: 649.00 USD
to the supplier
Mouse MMP-9
HUABIO
catalog: HA723788B
domestic rabbit monoclonal (PSH15-81)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse MMP-9 matched pair antibodies Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723785) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-9 protein (HA211045) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723787, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MMP-9 in mouse serum samples. Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] The concentrations of MMP-9 were measured in duplicates, interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-9 concentration was determined to be 370.1 ng/ml in mouse serum.

Interpolated concentrations of native MMP-9 in mouse LPS unstimulated and stimulated J774A.1 cell culture supernatant. Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] J774A.1 cells were stimulated with 2.5 µg/ml LPS or vehicle control and incubated for 1 day. The concentrations of MMP-9 measured in duplicate and interpolated from the MMP-9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50% and stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-9 concentration was determined to be 21,179 pg/ml in LPS stimulated J774A.1 cell culture supernatant and undetectable in the unstimulated J774A.1 control.
quantity: 100μl
price: 409.00 USD
to the supplier
Mouse MMP-9
HUABIO
catalog: HA723788H
domestic rabbit monoclonal (PSH15-81)
reactivity: mouse
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Mouse MMP-9 matched pair antibodies Capture: HA723785, Mouse MMP-9 Rabbit mAb [PSH15-80] Detector: HA723787, Mouse MMP-9 Rabbit mAb [PSH15-81] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723785) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-9 protein (HA211045) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA723787, HRP, 2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
MMP-9
HUABIO
catalog: HA750420
domestic rabbit monoclonal (JA80-73)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (HA750420) at 1/5,000 dilution. Lane 1: THP-1 whole cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 200nM TPA for 10 minutes whole cell lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 78 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750420) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MMP9 on different lysates with Rabbit anti-MMP9 antibody (HA750420) at 1/5,000 dilution. Lane 1: A549-si NT+TPA(80nM 24h) cell lysate, 15 µg/Lane Lane 2: A549-si MMP9#1+TPA(80nM 24h) cell lysate, 15 µg/Lane Lane 3: A549-si MMP9#2+TPA(80nM 24h) cell lysate, 15 µg/Lane Predicted band size: 78 kDa Observed band size: 78-92 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-69, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MMP-9 antibody (HA750420) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750420) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier