Lamin A + Lamin C
HUABIO
catalog: ET7110-12

domestic rabbit monoclonal (JE51-60)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Lamin A + Lamin C on different lysates with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: Mouse ovary tissue lysate (40 µg/Lane) Lane 5: Rat ovary tissue lysate (40 µg/Lane) Predicted band size: 74 kDa Observed band size: 70/65 kDa Exposure time: Lane 1-2: 25 seconds; Lane 3-5: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on different lysates with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Lamin A + Lamin C KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 63, 74 kDa Observed band size: 63, 74 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-12) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling Lamin A + Lamin C with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin A + Lamin C antibody (ET7110-12) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Lamin A + Lamin C
HUABIO
catalog: HA600093

mouse monoclonal (A6F5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Lamin A + Lamin C on different lysates with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600093) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on PC-12 cell lysates with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 4 minutes 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600093) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on different lysates with Mouse anti-Lamin A + Lamin C antibody (HA600093) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Lamin A + Lamin C KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600093) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

Lamin A + Lamin C
HUABIO
catalog: HA601274

mouse monoclonal (A6F5-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Lamin A + Lamin C on different lysates with Mouse anti-Lamin A + Lamin C antibody (HA601274) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601274) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on PC-12 cell lysates with Mouse anti-Lamin A + Lamin C antibody (HA601274) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 4 minutes 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601274) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on different lysates with Mouse anti-Lamin A + Lamin C antibody (HA601274) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Lamin A + Lamin C KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601274) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

Lamin A + Lamin C
HUABIO
catalog: HA610149

mouse monoclonal (A6F5-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Lamin A + Lamin C on different lysates with Mouse anti-Lamin A + Lamin C antibody (HA610149) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610149) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on PC-12 cell lysates with Mouse anti-Lamin A + Lamin C antibody (HA610149) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 4 minutes 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610149) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on different lysates with Mouse anti-Lamin A + Lamin C antibody (HA610149) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Lamin A + Lamin C KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610149) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier

Lamin A + Lamin C
HUABIO
catalog: HA722245

domestic rabbit monoclonal (PSH05-20)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Lamin A + Lamin C on different lysates with Rabbit anti-Lamin A + Lamin C antibody (HA722245) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 74 kDa Observed band size: 72/60 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722245) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on different lysates with Rabbit anti-Lamin A + Lamin C antibody (HA722245) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Lamin A + Lamin C KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 74 kDa Observed band size: 72/60 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722245) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling Lamin A + Lamin C with Rabbit anti-Lamin A + Lamin C antibody (HA722245) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin A + Lamin C antibody (HA722245) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Lamin A + Lamin C (S22)
HUABIO
catalog: HA723339

domestic rabbit monoclonal (PSH11-47)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Lamin A + Lamin C (S22) on different lysates with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA723339) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM paclitaxel for 20 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate Lane 5: HeLa treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lane 5: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 70/65 kDa Exposure time: Lane 1-2: 3 seconds; Lane 3-6: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723339) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA723339) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723339) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA723339) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723339) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Lamin A + Lamin C (S22)
HUABIO
catalog: HA723340

domestic rabbit monoclonal (PSH11-48)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Lamin A + Lamin C (S22) on different lysates with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA723340) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM paclitaxel for 20 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 4mM hydroxyurea for 20 hours cell lysate Lane 7: HeLa treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lane 8: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lane 9: C6 treated with 4mM hydroxyurea for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 70/65 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723340) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Lamin A + Lamin C
HUABIO
catalog: HA750991

domestic rabbit monoclonal (PSH05-20)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Lamin A + Lamin C on different lysates with Rabbit anti-Lamin A + Lamin C antibody (HA750991) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 74 kDa Observed band size: 72/60 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750991) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Lamin A + Lamin C on different lysates with Rabbit anti-Lamin A + Lamin C antibody (HA750991) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Lamin A + Lamin C KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 74 kDa Observed band size: 72/60 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750991) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells labeling Lamin A + Lamin C with Rabbit anti-Lamin A + Lamin C antibody (HA750991) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lamin A + Lamin C antibody (HA750991) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Lamin A + Lamin C (S22)
HUABIO
catalog: HA751409

domestic rabbit monoclonal (PSH11-47)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Lamin A + Lamin C (S22) on different lysates with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA751409) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM paclitaxel for 20 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate Lane 5: HeLa treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lane 5: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 70/65 kDa Exposure time: Lane 1-2: 3 seconds; Lane 3-6: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751409) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA751409) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751409) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA751409) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751409) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Lamin A + Lamin C (S22)
HUABIO
catalog: HA751410

domestic rabbit monoclonal (PSH11-48)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Lamin A + Lamin C (S22) on different lysates with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA751410) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM paclitaxel for 20 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 4mM hydroxyurea for 20 hours cell lysate Lane 7: HeLa treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lane 8: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lane 9: C6 treated with 4mM hydroxyurea for 20 hours cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 70/65 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751410) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier