DFNA5 / GSDME
HUABIO
catalog: EM1901-48

mouse monoclonal (A1H3)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of DFNA5 / GSDME on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate

ICC staining of DFNA5 / GSDME in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-DFNA5 / GSDME antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: EM1901-49

mouse monoclonal (A1H2)
reactivity: human
application: western blot

Western blot analysis of DFNA5 / GSDME on different lysates with Mouse anti-DFNA5 / GSDME antibody (EM1901-49) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SW480 cell lysate (negative) Lane 3: SGC-7901 cell lysate Lane 4: MCF7 cell lysate (negative) Lane 5: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-49) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: ER1901-12

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (ER1901-12) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Siha cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-12) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-DFNA5 / GSDME antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-12, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DFNA5 / GSDME antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-12, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA722836

domestic rabbit monoclonal (PSH07-48)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA722836) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: NCI-H226 cell lysate Lane 3: A549 cell lysate Lane 4: C2C12 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: Human brain tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722836) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA723113

domestic rabbit monoclonal (PSH09-51)
reactivity: human, mouse
application: western blot, immunoprecipitation

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723113) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723113) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723113) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (C-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723113) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA723113 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723113 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA723113 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA723113 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA723250

domestic rabbit monoclonal (PSH10-83)
reactivity: human
application: western blot

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723250) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723250) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA723251

domestic rabbit monoclonal (PSH10-84)
reactivity: human, mouse
application: western blot, immunoprecipitation

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723251) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723251) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA723251) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (N-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723251) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA723251 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723251 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA723251 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA723251 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 385.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA751169

domestic rabbit monoclonal (PSH07-48)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751169) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: NCI-H226 cell lysate Lane 3: A549 cell lysate Lane 4: C2C12 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: Human brain tissue lysate Lane 7: Mouse brain tissue lysate Lane 8: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751169) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA751298

domestic rabbit monoclonal (PSH09-51)
reactivity: human, mouse
application: western blot, immunoprecipitation

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751298) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751298) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (C-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 51 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA751298 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751298 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA751298 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA751298 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA751375

domestic rabbit monoclonal (PSH10-83)
reactivity: human
application: western blot

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751375) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751375) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

DFNA5 / GSDME
HUABIO
catalog: HA751376

domestic rabbit monoclonal (PSH10-84)
reactivity: human, mouse
application: western blot, immunoprecipitation

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751376) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: SW480 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751376) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody (HA751376) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with mouse GSDME (N-terminal) cell lysate Lane 3: 293T transfected with mouse GSDME (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751376) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

DFNA5 / GSDME was immunoprecipitated from 0.2 mg SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate with HA751376 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751376 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate (input) Lane 2: HA751376 IP in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Lane 3: Rabbit IgG instead of HA751376 in SH-SY5Y treated with 4μg/mL Doxorubicin for 24 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801
quantity: 100μl
price: 649.00 USD
to the supplier