domestic rabbit monoclonal (PSH05-55)
reactivity: mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722302) at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 30 µg/Lane. Predicted band size: 44 kDa Observed band size: 100-120 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722302) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Dorsal root ganglion Sample: Frozen section Antibody concentration: 1: 500 Date by conrtesy of: Mr. Rongyao Ye, School of Basic Medical Sicences, Zhejiang University
Application: IHC-Fr Species: Mouse Site: Colon Sample: Frozen section Antibody concentration: 1: 500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-39)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: HeLa cell lysate Lane 7: HeLa cell lysate treated with deglycosylation Lane 8: NIH/3T3 cell lysate Lane 9: NIH/3T3 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 120/44 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: L6 cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Predicted band size: 44 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Kidney Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-55)
reactivity: mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA751017) at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 30 µg/Lane. Predicted band size: 44 kDa Observed band size: 100-120 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751017) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Dorsal root ganglion Sample: Frozen section Antibody concentration: 1: 500 Date by conrtesy of: Mr. Rongyao Ye, School of Basic Medical Sicences, Zhejiang University
Application: IHC-Fr Species: Mouse Site: Colon Sample: Frozen section Antibody concentration: 1: 500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-39)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA751160) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: HeLa cell lysate Lane 7: HeLa cell lysate treated with deglycosylation Lane 8: NIH/3T3 cell lysate Lane 9: NIH/3T3 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 120/44 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA751160) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: L6 cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Predicted band size: 44 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Kidney Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
quantity: 100μl
price: 649.00 USD
to the supplier