DDIT3
HUABIO
catalog: ER1803-20

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry

Western blot analysis of DDIT3 on different lysates using anti-DDIT3 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis tissue Lane 2: LOVO Lane 3: Mouse brain tissue

Flow cytometric analysis of HepG2 cells with DDIT3 antibody at 1/50 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
quantity: 100μl
price: 330 USD
to the supplier

DDIT3
HUABIO
catalog: ET1703-05

domestic rabbit monoclonal (JM10-31)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (ET1703-05) at 1/5,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-05) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (ET1703-05) at 1/1,000 dilution. Lane 1: SW480-si NT cell lysate Lane 2: SW480-si DDIT3 cell lysate Lysates/proteins at 3 µg/Lane. Predicted band size: 19 kDa Observed band size: 25 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. ET1703-05 was shown to specifically react with DDIT3 in SW480-si NT cells. Weakened band was observed when SW480-si DDIT3 sample was tested. SW480-si NT and SW480-si DDIT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-05, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate Predicted band size: 19 kDa Observed band size: 25 kDa
quantity: 100μl
price: 385.00 USD
to the supplier

DDIT3
HUABIO
catalog: HA722854

domestic rabbit monoclonal (PSH07-65)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (HA722854) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 2μg/mL tunicamycin for 8 hours cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 2μg/mL thapsigargin for 8 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 2μg/mL tunicamycin for 8 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-6: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722854) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells treated with 2μg/mL tunicamycin for 8 hours labeling DDIT3 with Rabbit anti-DDIT3 antibody (HA722854) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDIT3 antibody (HA722854) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of C2C12 cells treated with 2μg/mL thapsigargin for 8 hours labeling DDIT3 with Rabbit anti-DDIT3 antibody (HA722854) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDIT3 antibody (HA722854) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

DDIT3
HUABIO
catalog: HA750369

domestic rabbit monoclonal (JM10-31)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (HA750369) at 1/5,000 dilution. Lane 1: SW480 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750369) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (HA750369) at 1/1,000 dilution. Lane 1: SW480-si NT cell lysate Lane 2: SW480-si DDIT3 cell lysate Lysates/proteins at 3 µg/Lane. Predicted band size: 19 kDa Observed band size: 25 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. ET1703-05 was shown to specifically react with DDIT3 in SW480-si NT cells. Weakened band was observed when SW480-si DDIT3 sample was tested. SW480-si NT and SW480-si DDIT3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-05, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750369, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate Predicted band size: 19 kDa Observed band size: 25 kDa
quantity: 100μl
price: 649.00 USD
to the supplier

DDIT3
HUABIO
catalog: HA751178

domestic rabbit monoclonal (PSH07-65)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of DDIT3 on different lysates with Rabbit anti-DDIT3 antibody (HA751178) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 2μg/mL tunicamycin for 8 hours cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 2μg/mL thapsigargin for 8 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 2μg/mL tunicamycin for 8 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 27 kDa Exposure time: Lane 1-2: 3 minutes; Lane 3-6: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751178) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HeLa cells treated with 2μg/mL tunicamycin for 8 hours labeling DDIT3 with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of C2C12 cells treated with 2μg/mL thapsigargin for 8 hours labeling DDIT3 with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDIT3 antibody (HA751178) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier

Anti-DDIT3 Antibody [JM10-31]
HUABIO
catalog: ET1703-05TR

domestic rabbit monoclonal (JM10-31)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

quantity: 20 uL
price: 99 USD
to the supplier