domestic rabbit monoclonal (PSH07-42)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA722830) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2:NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 3: C2C12 cell lysate (negative) (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (30 µg/Lane) Lane 5: Mouse colon tissue lysate (30 µg/Lane) Predicted band size: 129 kDa Observed band size: 140 kDa Exposure time: Lane1-3: 3 minutes ; Lane 4-5: 25 seconds；ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of frozen mouse colon tissue with Rabbit anti-CD11c antibody (HA722830) at 1/1,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722830, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-CD11c antibody (HA722830) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722830, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit polyclonal (PSH13-25)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA723512) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse colon tissue lysate (40 µg/Lane) Lane 6: Mouse spleen tissue lysate (40 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 129 kDa Observed band size: 140 kDa Exposure time: Lane 1-2: 59 seconds; Lane 3-5: 25 seconds; Lane 6: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723512) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Rabbit anti-CD11c antibody (HA723512) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723512) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-42)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA751163) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2:NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 3: C2C12 cell lysate (negative) (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (30 µg/Lane) Lane 5: Mouse colon tissue lysate (30 µg/Lane) Predicted band size: 129 kDa Observed band size: 140 kDa Exposure time: Lane1-3: 3 minutes ; Lane 4-5: 25 seconds；ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of frozen mouse colon tissue with Rabbit anti-CD11c antibody (HA751163) at 1/1,000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751163, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-CD11c antibody (HA751163) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751163, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal (PSH13-25)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA751461) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C2C12 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse colon tissue lysate (40 µg/Lane) Lane 6: Mouse spleen tissue lysate (40 µg/Lane) Lysates/proteins at 20 µg/Lane. Predicted band size: 129 kDa Observed band size: 140 kDa Exposure time: Lane 1-2: 59 seconds; Lane 3-5: 25 seconds; Lane 6: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751461) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Rabbit anti-CD11c antibody (HA751461) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751461) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal
reactivity: mouse
reactivity: mouse
mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS084) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
mIHC analysis of mouse pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS084) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS084) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 289.00 USD
to the supplier