IL-6
HUABIO
catalog: EM1701-45

mouse monoclonal (1-6)
reactivity: mouse, rat
application: western blot

Western blot analysis of IL6 on mouse IL6 recombinant protein using anti-IL6 antibody at 1/1,000 dilution.

Immunocytochemistry analysis of EL4 cells labeling IL-6 with Mouse anti-IL-6 antibody (EM1701-45) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-IL-6 antibody (EM1701-45) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 360.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA721411

domestic rabbit monoclonal (PSH0-58)
reactivity: mouse
application: ELISA

Standard curve of mouse IL-6 matched pair antibodies: Sandwich ELISA analysis of mouse IL-6 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody PSH0-58 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-6 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody PSH0-60-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Standard curve of mouse IL-6 matched pair antibodies: Sandwich ELISA analysis of mouse IL-6 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody PSH0-58 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-6 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody PSH0-59-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA721412

domestic rabbit monoclonal (PSH0-59)
reactivity: mouse
application: ELISA

Standard curve of mouse IL-6 matched pair antibodies: Sandwich ELISA analysis of mouse IL-6 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody PSH0-58 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-6 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody PSH0-59-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA721413

domestic rabbit monoclonal (PSH0-60)
reactivity: mouse
application: ELISA

Standard curve of mouse IL-6 matched pair antibodies: Sandwich ELISA analysis of mouse IL-6 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody PSH0-58 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-6 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody PSH0-60-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA721600

domestic rabbit monoclonal (PS01-74)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-6 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721600) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-6 protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721601, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA721601

domestic rabbit monoclonal (PS01-83)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-6 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721600) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-6 protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721601, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA721602

domestic rabbit monoclonal (PS01-83)
reactivity: mouse
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Mouse IL-6 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721600) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-6 protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721602, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA722646B

domestic rabbit monoclonal (PSH0-59)
reactivity: mouse
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA722647B

domestic rabbit monoclonal (PSH0-60)
reactivity: mouse
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA723642

domestic rabbit monoclonal (PSH14-47)
reactivity: mouse
application: ELISA

quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: HA723643B

domestic rabbit monoclonal (PS01-74)
reactivity: mouse
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-6
HUABIO
catalog: M1303-2

mouse monoclonal (3-A4)
reactivity: mouse
conjugate: HRP
application: western blot, ELISA

Western blot analysis on bioactive mouse IL-6 protein using anti-mouse Interleukin 6 Conjugated HRP Mouse mAb (Cat. # M1303-2).
quantity: 100μl
price: 384.00 USD
to the supplier

IL-6
HUABIO
catalog: M1303-3

mouse monoclonal (3-A4)
reactivity: mouse
application: western blot, ELISA, flow cytometry

Western blot analysis on bioactive mouse IL-6 protein using anti-IL-6 Mouse mAb (Cat. # M1303-3).
quantity: 100μl
price: 360.00 USD
to the supplier

IL-6
HUABIO
catalog: R1203-2

domestic rabbit polyclonal
reactivity: mouse
application: western blot, ELISA

Western Blot analysis of recombinant murine IL-6 using rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier

IL-6
HUABIO
catalog: R1412-2

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of IL-6 on recombinant murine Interleukin-6 protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1412-2, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

ICC staining of IL-6 in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1412-2, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1412-2, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier