Mouse IL-4
HUABIO
catalog: HA722168

domestic rabbit monoclonal (PSH04-68)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722168) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-4 protein (HA210902) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA722169, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-4
HUABIO
catalog: HA722169

domestic rabbit monoclonal (PSH04-69)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722168) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-4 protein (HA210902) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA722169, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Mouse IL-4
HUABIO
catalog: HA722262

domestic rabbit monoclonal (PSH05-33)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722262) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-4 protein (HA210741) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722263, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked IL-4 in mouse cell culture media samples. The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μg
price: 800.00 USD
to the supplier

Mouse IL-4
HUABIO
catalog: HA722263

domestic rabbit monoclonal (PSH05-34)
reactivity: mouse
application: ELISA

Sandwich ELISA analysis of Mouse IL-4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722262) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-4 protein (HA210741) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722263, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of spiked IL-4 in mouse cell culture media samples. The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μg
price: 800.00 USD
to the supplier

Mouse IL-4
HUABIO
catalog: HA722597B

domestic rabbit monoclonal (PSH05-34)
reactivity: mouse
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

Mouse IL-4
HUABIO
catalog: HA722607B

domestic rabbit monoclonal (PSH04-69)
reactivity: mouse
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier